Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3182981..3183668 Replicon chromosome
Accession NZ_CP023575
Organism Mycobacterium tuberculosis strain CSV5769

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag CP431_RS15685 Protein ID WP_003414624.1
Coordinates 3183225..3183668 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag CP431_RS15680 Protein ID WP_003414620.1
Coordinates 3182981..3183238 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP431_RS15660 3178301..3179155 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
CP431_RS15665 3179211..3180374 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
CP431_RS15670 3180391..3181605 - 1215 WP_003899518.1 zinc metalloprotease Rip -
CP431_RS15675 3181613..3182854 - 1242 WP_031658947.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
CP431_RS15680 3182981..3183238 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
CP431_RS15685 3183225..3183668 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
CP431_RS15690 3183748..3184410 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
CP431_RS15695 3184506..3184697 + 192 WP_031658948.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
CP431_RS15700 3185029..3186777 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
CP431_RS15705 3186873..3187454 + 582 WP_003414644.1 fasciclin domain-containing protein -
CP431_RS15710 3187554..3187820 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T81839 WP_003414624.1 NZ_CP023575:3183225-3183668 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T81839 NZ_CP023575:3183225-3183668 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT81839 WP_003414620.1 NZ_CP023575:3182981-3183238 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT81839 NZ_CP023575:3182981-3183238 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References