Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3110001..3110571 Replicon chromosome
Accession NZ_CP023575
Organism Mycobacterium tuberculosis strain CSV5769

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CP431_RS15315 Protein ID WP_003414166.1
Coordinates 3110001..3110357 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CP431_RS15320 Protein ID WP_003901465.1
Coordinates 3110341..3110571 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP431_RS15295 3105453..3107141 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
CP431_RS15300 3107145..3107471 - 327 WP_003414157.1 hypothetical protein -
CP431_RS15305 3107644..3108231 + 588 WP_003914429.1 DUF3558 family protein -
CP431_RS15310 3108250..3109899 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
CP431_RS15315 3110001..3110357 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CP431_RS15320 3110341..3110571 - 231 WP_003901465.1 antitoxin MazE Antitoxin
CP431_RS15325 3110614..3111657 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
CP431_RS15330 3111848..3112123 + 276 WP_003911993.1 DUF1778 domain-containing protein -
CP431_RS21555 3112299..3112553 - 255 WP_003917684.1 hypothetical protein -
CP431_RS15340 3112701..3113105 + 405 WP_009938577.1 hypothetical protein -
CP431_RS15345 3113102..3113293 + 192 WP_003414184.1 hypothetical protein -
CP431_RS15350 3113357..3114646 + 1290 Protein_2902 transposase family protein -
CP431_RS15355 3114880..3115137 + 258 WP_003899489.1 hypothetical protein -
CP431_RS15360 3115242..3115553 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T81835 WP_003414166.1 NZ_CP023575:c3110357-3110001 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T81835 NZ_CP023575:c3110357-3110001 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT81835 WP_003901465.1 NZ_CP023575:c3110571-3110341 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT81835 NZ_CP023575:c3110571-3110341 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References