Detailed information of TA system TA081829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2868450..2869081
• Replicon: chromosome
• Accession: NZ_CP023575
• Organism: Mycobacterium tuberculosis strain CSV5769

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: CP431_RS13950
• Protein ID: WP_003413174.1
• Coordinates: 2868704..2869081 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: CP431_RS13945
• Protein ID: WP_003413167.1
• Coordinates: 2868450..2868707 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP431_RS13910	2864364..2864678	+	315	WP_009937839.1	hypothetical protein	-
CP431_RS13915	2864974..2866185	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
CP431_RS13925	2866312..2866971	+	660	WP_003900846.1	LppA family lipoprotein	-
CP431_RS13930	2866968..2867630	+	663	WP_003900848.1	hypothetical protein	-
CP431_RS13935	2867627..2867905	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
CP431_RS13940	2867998..2868411	+	414	WP_003413164.1	PIN domain nuclease	-
CP431_RS13945	2868450..2868707	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP431_RS13950	2868704..2869081	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP431_RS13955	2869097..2869471	-	375	WP_003413177.1	hypothetical protein	-
CP431_RS13960	2869571..2869966	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
CP431_RS13965	2869963..2870208	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
CP431_RS13975	2870619..2871038	-	420	WP_003413190.1	A24 family peptidase	-
CP431_RS13980	2871050..2871859	-	810	WP_003413193.1	shikimate dehydrogenase	-
CP431_RS13985	2871856..2873109	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
CP431_RS13990	2873102..2873614	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T81829 WP_003413174.1 NZ_CP023575:2868704-2869081 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T81829 NZ_CP023575:2868704-2869081 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT81829 WP_003413167.1 NZ_CP023575:2868450-2868707 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT81829 NZ_CP023575:2868450-2868707 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5