81712

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3069939..3070618 Replicon chromosome
Accession NZ_CP023573
Organism Mycobacterium tuberculosis strain CSV4519

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag CP429_RS15110 Protein ID WP_003414059.1
Coordinates 3069939..3070355 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag CP429_RS15115 Protein ID WP_003414061.1
Coordinates 3070352..3070618 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP429_RS15090 3065991..3066893 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
CP429_RS15095 3066962..3067714 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
CP429_RS15100 3067958..3068233 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
CP429_RS15105 3068230..3069852 - 1623 WP_003906897.1 type I restriction-modification system subunit M -
CP429_RS15110 3069939..3070355 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
CP429_RS15115 3070352..3070618 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP429_RS15120 3070644..3071039 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
CP429_RS15125 3071036..3071305 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
CP429_RS15130 3071315..3072409 - 1095 WP_003414068.1 restriction endonuclease subunit S -
CP429_RS15135 3072406..3072825 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
CP429_RS15140 3072899..3073378 - 480 WP_003414073.1 dihydrofolate reductase -
CP429_RS15145 3073449..3074249 - 801 WP_009939104.1 thymidylate synthase -
CP429_RS15150 3074405..3075142 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T81712 WP_003414059.1 NZ_CP023573:c3070355-3069939 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T81712 NZ_CP023573:c3070355-3069939 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT81712 WP_003414061.1 NZ_CP023573:c3070618-3070352 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT81712 NZ_CP023573:c3070618-3070352 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References