Detailed information of TA system TA081694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194475..2195178
• Replicon: chromosome
• Accession: NZ_CP023573
• Organism: Mycobacterium tuberculosis strain CSV4519

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: CP429_RS10570
• Protein ID: WP_003409778.1
• Coordinates: 2194475..2194804 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: CP429_RS10575
• Protein ID: WP_003409780.1
• Coordinates: 2194801..2195178 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP429_RS10550	2190858..2191928	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
CP429_RS10555	2191925..2192440	+	516	WP_003409718.1	flavin reductase family protein	-
CP429_RS10560	2192437..2193498	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
CP429_RS10565	2193495..2194265	+	771	WP_003409775.1	SDR family oxidoreductase	-
CP429_RS10570	2194475..2194804	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CP429_RS10575	2194801..2195178	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
CP429_RS10580	2195175..2195765	-	591	WP_003409784.1	SEC-C domain-containing protein	-
CP429_RS10585	2195820..2197184	+	1365	WP_003906706.1	HNH endonuclease	-
CP429_RS10590	2197339..2197791	-	453	WP_003899095.1	lipoprotein	-
CP429_RS10595	2197855..2198256	+	402	WP_003409869.1	hypothetical protein	-
CP429_RS10600	2198249..2198431	-	183	WP_003409870.1	hypothetical protein	-
CP429_RS10605	2198545..2198895	-	351	WP_003899096.1	hypothetical protein	-
CP429_RS10610	2198906..2199808	-	903	WP_003899097.1	hypothetical protein	-
CP429_RS10615	2199829..2200020	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T81694 WP_003409778.1 NZ_CP023573:c2194804-2194475 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T81694 NZ_CP023573:c2194804-2194475 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT81694 WP_003409780.1 NZ_CP023573:c2195178-2194801 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT81694 NZ_CP023573:c2195178-2194801 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8