Detailed information of TA system TA081661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4749137..4749753
• Replicon: chromosome
• Accession: NZ_CP023569
• Organism: Enterobacter hormaechei strain BW

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CPT31_RS22505
• Protein ID: WP_017382676.1
• Coordinates: 4749137..4749508 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: CPT31_RS22510
• Protein ID: WP_015569912.1
• Coordinates: 4749511..4749753 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPT31_RS22490	4746637..4747539	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
CPT31_RS22495	4747536..4748171	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
CPT31_RS22500	4748168..4749097	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
CPT31_RS22505	4749137..4749508	-	372	WP_017382676.1	type II toxin-antitoxin system VapC family toxin	Toxin
CPT31_RS22510	4749511..4749753	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
CPT31_RS22515	4749952..4750872	+	921	WP_017382675.1	alpha/beta hydrolase	-
CPT31_RS22520	4750881..4751822	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
CPT31_RS22525	4751867..4752304	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CPT31_RS22530	4752301..4753182	-	882	WP_003861949.1	virulence factor BrkB family protein	-
CPT31_RS22535	4753176..4753775	-	600	WP_003861947.1	glucose-1-phosphatase	-
CPT31_RS22540	4753894..4754694	-	801	WP_017382673.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13737.88 Da
• Isoelectric Point: 6.4882

>T81661 WP_017382676.1 NZ_CP023569:c4749508-4749137 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T81661 NZ_CP023569:c4749508-4749137 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT81661 WP_015569912.1 NZ_CP023569:c4749753-4749511 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT81661 NZ_CP023569:c4749753-4749511 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3