Detailed information of TA system TA081643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2729167..2729683
• Replicon: chromosome
• Accession: NZ_CP023567
• Organism: Erwinia pyrifoliae strain EpK1/15

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D2T7S6
• Locus tag: CPI84_RS13235
• Protein ID: WP_012669379.1
• Coordinates: 2729399..2729683 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D2T7S5
• Locus tag: CPI84_RS13230
• Protein ID: WP_012669378.1
• Coordinates: 2729167..2729409 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPI84_RS13215	2724429..2724830	-	402	WP_012669375.1	helix-turn-helix domain-containing protein	-
CPI84_RS13220	2724943..2727636	+	2694	WP_012669376.1	toprim domain-containing protein	-
CPI84_RS13225	2727851..2728906	+	1056	WP_012669377.1	site-specific tyrosine recombinase XerC	-
CPI84_RS13230	2729167..2729409	+	243	WP_012669378.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CPI84_RS13235	2729399..2729683	+	285	WP_012669379.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CPI84_RS19890	2730185..2730361	-	177	WP_157861020.1	hypothetical protein	-
CPI84_RS13245	2730496..2730678	+	183	WP_081441632.1	hypothetical protein	-
CPI84_RS13250	2730743..2731177	+	435	WP_071819801.1	hypothetical protein	-
CPI84_RS13255	2731188..2731571	+	384	WP_071819843.1	immunity 50 family protein	-
CPI84_RS13265	2732866..2733084	-	219	WP_041473981.1	SymE family type I addiction module toxin	-
CPI84_RS13270	2733138..2733554	+	417	WP_014538693.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2715902..2733317	17415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11123.03 Da
• Isoelectric Point: 10.3769

>T81643 WP_012669379.1 NZ_CP023567:2729399-2729683 [Erwinia pyrifoliae]
MSYELVFDPRALKEWKKLGATVREQFKKKLAEVLVNPRVESARLREYPDCYKIKLKSSGYRLVYQVQDEQLVVFVVATGK
RERLQVYRDAGKRL

Nucleotide

• Length: 285 bp

>T81643 NZ_CP023567:2729399-2729683 [Erwinia pyrifoliae]
ATGAGCTATGAACTGGTCTTCGATCCCAGAGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAGCAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCCGAGGGTGGAATCTGCCAGGTTGAGAGAGTACCCGGACTGCTACAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTTGTCTTCGTTGTGGCGACAGGAAAA
AGAGAAAGGTTACAGGTGTATCGTGACGCAGGAAAGCGCCTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9070.45 Da
• Isoelectric Point: 4.6310

>AT81643 WP_012669378.1 NZ_CP023567:2729167-2729409 [Erwinia pyrifoliae]
MATLNVRLDDKLKSKAYAVLDELDVSPTEAVRLLFQYIAENERLPLKTLTVSDEEDALLKTVRERLASPQKGIRVTLDEL

Nucleotide

• Length: 243 bp

>AT81643 NZ_CP023567:2729167-2729409 [Erwinia pyrifoliae]
ATGGCAACATTAAACGTGCGGCTGGATGACAAGCTGAAAAGCAAAGCTTACGCAGTCCTGGATGAACTGGATGTATCACC
AACAGAGGCGGTCCGGCTGCTGTTCCAGTATATAGCGGAAAATGAACGTCTGCCGCTGAAAACGCTGACGGTCAGCGATG
AAGAGGACGCGCTGCTCAAAACGGTGCGCGAAAGGCTGGCATCGCCACAGAAGGGAATAAGGGTCACGCTGGATGAGCTA
TGA

Structures

Toxin

• AlphaFold DB: D2T7S6

Antitoxin

• AlphaFold DB: D2T7S5