Detailed information of TA system TA081642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2721518..2722034
• Replicon: chromosome
• Accession: NZ_CP023567
• Organism: Erwinia pyrifoliae strain EpK1/15

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D2T7S6
• Locus tag: CPI84_RS13185
• Protein ID: WP_012669379.1
• Coordinates: 2721750..2722034 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D2T7S5
• Locus tag: CPI84_RS13180
• Protein ID: WP_012669378.1
• Coordinates: 2721518..2721760 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPI84_RS13165	2716795..2717196	-	402	WP_012669375.1	helix-turn-helix domain-containing protein	-
CPI84_RS13170	2717309..2719987	+	2679	WP_104945112.1	toprim domain-containing protein	-
CPI84_RS13175	2720202..2721257	+	1056	WP_012669377.1	site-specific tyrosine recombinase XerC	-
CPI84_RS13180	2721518..2721760	+	243	WP_012669378.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CPI84_RS13185	2721750..2722034	+	285	WP_012669379.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CPI84_RS19885	2722536..2722712	-	177	WP_157861020.1	hypothetical protein	-
CPI84_RS13195	2722847..2723029	+	183	WP_081441632.1	hypothetical protein	-
CPI84_RS13200	2723067..2723531	+	465	Protein_2484	HNH endonuclease	-
CPI84_RS13205	2723536..2723988	+	453	WP_012669373.1	SMI1/KNR4 family protein	-
CPI84_RS13210	2724048..2724350	-	303	WP_012669374.1	SymE family type I addiction module toxin	-
CPI84_RS13215	2724429..2724830	-	402	WP_012669375.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2715902..2733317	17415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11123.03 Da
• Isoelectric Point: 10.3769

>T81642 WP_012669379.1 NZ_CP023567:2721750-2722034 [Erwinia pyrifoliae]
MSYELVFDPRALKEWKKLGATVREQFKKKLAEVLVNPRVESARLREYPDCYKIKLKSSGYRLVYQVQDEQLVVFVVATGK
RERLQVYRDAGKRL

Nucleotide

• Length: 285 bp

>T81642 NZ_CP023567:2721750-2722034 [Erwinia pyrifoliae]
ATGAGCTATGAACTGGTCTTCGATCCCAGAGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAGCAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCCGAGGGTGGAATCTGCCAGGTTGAGAGAGTACCCGGACTGCTACAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTTGTCTTCGTTGTGGCGACAGGAAAA
AGAGAAAGGTTACAGGTGTATCGTGACGCAGGAAAGCGCCTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9070.45 Da
• Isoelectric Point: 4.6310

>AT81642 WP_012669378.1 NZ_CP023567:2721518-2721760 [Erwinia pyrifoliae]
MATLNVRLDDKLKSKAYAVLDELDVSPTEAVRLLFQYIAENERLPLKTLTVSDEEDALLKTVRERLASPQKGIRVTLDEL

Nucleotide

• Length: 243 bp

>AT81642 NZ_CP023567:2721518-2721760 [Erwinia pyrifoliae]
ATGGCAACATTAAACGTGCGGCTGGATGACAAGCTGAAAAGCAAAGCTTACGCAGTCCTGGATGAACTGGATGTATCACC
AACAGAGGCGGTCCGGCTGCTGTTCCAGTATATAGCGGAAAATGAACGTCTGCCGCTGAAAACGCTGACGGTCAGCGATG
AAGAGGACGCGCTGCTCAAAACGGTGCGCGAAAGGCTGGCATCGCCACAGAAGGGAATAAGGGTCACGCTGGATGAGCTA
TGA

Structures

Toxin

• AlphaFold DB: D2T7S6

Antitoxin

• AlphaFold DB: D2T7S5