Detailed information of TA system TA081632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 499665..500315
• Replicon: chromosome
• Accession: NZ_CP023565
• Organism: Ligilactobacillus murinus strain CR147

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CPS94_RS02475
• Protein ID: WP_076150269.1
• Coordinates: 499665..499853 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CPS94_RS02480
• Protein ID: WP_167397199.1
• Coordinates: 499932..500315 (+)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPS94_RS02460	498063..498545	+	483	WP_112195360.1	tyrosine-type recombinase/integrase	-
CPS94_RS02465	498860..499114	+	255	WP_112195358.1	hypothetical protein	-
CPS94_RS02470	499155..499412	+	258	WP_112195356.1	hypothetical protein	-
CPS94_RS02475	499665..499853	+	189	WP_076150269.1	type II toxin-antitoxin system HicA family toxin	Toxin
CPS94_RS02480	499932..500315	+	384	WP_167397199.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CPS94_RS02485	500607..500734	-	128	Protein_487	AraC family ligand binding domain-containing protein	-
CPS94_RS02490	500811..501248	-	438	WP_112195353.1	Rrf2 family transcriptional regulator	-
CPS94_RS02495	501510..502841	+	1332	WP_112195351.1	M20/M25/M40 family metallo-hydrolase	-
CPS94_RS02500	502971..504665	+	1695	WP_112195349.1	oleate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	cpsA	498063..543076	45013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-120)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7244.60 Da
• Isoelectric Point: 10.7107

>T81632 WP_076150269.1 NZ_CP023565:499665-499853 [Ligilactobacillus murinus]
MPLTGKDMLKLLKKNGWKEIRVTGSHHVMEKDSKQIIVPVHDNKDLRKGLEQRILKDAELKR

Nucleotide

• Length: 189 bp

>T81632 NZ_CP023565:499665-499853 [Ligilactobacillus murinus]
ATGCCACTTACAGGTAAAGATATGCTCAAACTTCTTAAAAAGAATGGCTGGAAAGAAATACGAGTTACTGGTTCTCACCA
TGTGATGGAAAAAGATAGTAAACAAATTATTGTTCCAGTTCATGATAATAAAGACCTGCGCAAGGGACTTGAGCAACGTA
TCTTGAAAGACGCTGAACTTAAGCGTTAA

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 14123.11 Da
• Isoelectric Point: 4.2788

>AT81632 WP_167397199.1 NZ_CP023565:499932-500315 [Ligilactobacillus murinus]
MAYPAIFKQEEEGGYFIEFPDLDGVYTGINEDDIPFGLEMASEALGIMLAEFIQNGKKFNDPSPVNAIEHDKNSFVTLVA
VDVAKYFEKDKLVKKTLSIPAWVDERGKKLGLNFSALLTKAVLETSE

Nucleotide

• Length: 384 bp

>AT81632 NZ_CP023565:499932-500315 [Ligilactobacillus murinus]
ATGGCATATCCTGCTATTTTTAAGCAAGAAGAAGAAGGGGGATACTTTATTGAGTTCCCTGATTTGGACGGTGTATATAC
TGGGATAAATGAAGATGACATACCTTTTGGATTAGAGATGGCCAGTGAAGCACTAGGTATCATGTTAGCTGAATTTATTC
AAAACGGTAAGAAATTTAATGATCCATCACCTGTAAATGCTATTGAGCATGATAAAAATAGCTTTGTAACATTAGTAGCA
GTAGATGTTGCTAAATATTTTGAAAAGGATAAGCTTGTTAAAAAAACGTTATCTATTCCAGCTTGGGTAGATGAGAGAGG
CAAGAAGTTAGGACTTAATTTTTCAGCTTTGTTGACTAAAGCAGTTCTTGAAACATCAGAATAA