Detailed information of TA system TA081629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 1641848..1642363
• Replicon: chromosome
• Accession: NZ_CP023563
• Organism: Brachybacterium vulturis strain VM2412

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CFK38_RS07380
• Protein ID: WP_096802495.1
• Coordinates: 1642109..1642363 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: CFK38_RS07375
• Protein ID: WP_096802494.1
• Coordinates: 1641848..1642108 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFK38_RS07335	1637174..1637962	+	789	WP_096802487.1	hypothetical protein	-
CFK38_RS07340	1638024..1639109	+	1086	WP_096802488.1	redox-regulated ATPase YchF	-
CFK38_RS07345	1639143..1639424	+	282	WP_096802489.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CFK38_RS07350	1639399..1639719	+	321	WP_096802490.1	HigA family addiction module antidote protein	-
CFK38_RS07355	1639716..1640540	+	825	WP_096802491.1	alpha/beta hydrolase	-
CFK38_RS07365	1641117..1641359	+	243	WP_096802492.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CFK38_RS07370	1641356..1641613	+	258	WP_096802493.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CFK38_RS07375	1641848..1642108	+	261	WP_096802494.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CFK38_RS07380	1642109..1642363	+	255	WP_096802495.1	Txe/YoeB family addiction module toxin	Toxin
CFK38_RS07385	1642597..1642926	+	330	WP_096802496.1	nucleotidyltransferase domain-containing protein	-
CFK38_RS07390	1642923..1643168	+	246	Protein_1453	DUF86 domain-containing protein	-
CFK38_RS07395	1643329..1643562	+	234	WP_096802497.1	Arc family DNA-binding protein	-
CFK38_RS07400	1643559..1643984	+	426	WP_096804262.1	type II toxin-antitoxin system VapC family toxin	-
CFK38_RS07405	1644132..1644707	-	576	WP_096802498.1	hypothetical protein	-
CFK38_RS07410	1644817..1645614	-	798	WP_157773402.1	hypothetical protein	-
CFK38_RS07415	1645625..1645978	-	354	WP_096802500.1	PadR family transcriptional regulator	-
CFK38_RS07420	1646093..1646701	+	609	WP_096802501.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (3-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-70)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9986.28 Da
• Isoelectric Point: 5.6193

>T81629 WP_096802495.1 NZ_CP023563:1642109-1642363 [Brachybacterium vulturis]
VRLVWDESAWEDYEHWQTADRKILKRINSLIDACLREPFSGIGKPEQLKYGAQGAWSRRITDEHRLVCLVDDEDLVILQA
RYHY

Nucleotide

• Length: 255 bp

>T81629 NZ_CP023563:1642109-1642363 [Brachybacterium vulturis]
GTGCGTCTGGTCTGGGACGAGTCGGCGTGGGAGGACTACGAGCACTGGCAGACCGCCGACCGGAAGATCCTGAAGCGCAT
CAACTCGCTGATCGACGCCTGCCTGCGCGAACCGTTCTCCGGGATCGGCAAGCCCGAGCAGCTGAAGTACGGAGCGCAGG
GTGCCTGGTCGCGGCGCATCACCGATGAGCACCGGCTGGTCTGCCTCGTGGACGACGAGGACCTCGTGATCCTGCAGGCC
CGCTATCACTACTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9717.83 Da
• Isoelectric Point: 4.7010

>AT81629 WP_096802494.1 NZ_CP023563:1641848-1642108 [Brachybacterium vulturis]
MSISASEARKTLFPLIERVNEDHEAVEIVSRKGNAVLMPADEYAAWQETAYLFRSPANARRLLDAYDRARAGKVEVHDLD
RADEEA

Nucleotide

• Length: 261 bp

>AT81629 NZ_CP023563:1641848-1642108 [Brachybacterium vulturis]
ATGTCCATCAGCGCAAGCGAGGCGCGCAAGACCCTCTTCCCTCTCATCGAGCGCGTGAACGAGGACCACGAGGCCGTCGA
GATCGTCTCTCGCAAGGGCAACGCGGTTCTCATGCCGGCCGACGAGTACGCCGCGTGGCAGGAGACCGCCTACCTGTTCC
GCTCGCCGGCCAACGCCCGCCGCCTGCTCGATGCCTACGACCGTGCCCGCGCCGGAAAGGTCGAGGTCCACGACCTCGAT
CGCGCAGACGAGGAAGCCTGA