Detailed information of TA system TA081624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2414339..2414868
• Replicon: chromosome
• Accession: NZ_CP023561
• Organism: Staphylococcus aureus strain TF3198

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CPC18_RS12470
• Protein ID: WP_000621175.1
• Coordinates: 2414339..2414701 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CPC18_RS12475
• Protein ID: WP_000948331.1
• Coordinates: 2414698..2414868 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPC18_RS12445	2411317..2412087	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
CPC18_RS12450	2412062..2412541	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
CPC18_RS12455	2412543..2412869	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CPC18_RS12460	2412988..2413989	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CPC18_RS12470	2414339..2414701	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CPC18_RS12475	2414698..2414868	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CPC18_RS12480	2414953..2416101	-	1149	WP_001281145.1	alanine racemase	-
CPC18_RS12485	2416167..2416526	-	360	WP_000581200.1	holo-ACP synthase	-
CPC18_RS12490	2416530..2417021	-	492	WP_001205910.1	PH domain-containing protein	-
CPC18_RS12495	2417008..2418591	-	1584	WP_001294626.1	PH domain-containing protein	-
CPC18_RS12500	2418584..2419063	-	480	WP_001287088.1	hypothetical protein	-
CPC18_RS12505	2419272..2419832	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T81624 WP_000621175.1 NZ_CP023561:c2414701-2414339 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T81624 NZ_CP023561:c2414701-2414339 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT81624 WP_000948331.1 NZ_CP023561:c2414868-2414698 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT81624 NZ_CP023561:c2414868-2414698 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1