Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 29873..30398 Replicon plasmid pFORC43
Accession NZ_CP023552
Organism Escherichia coli strain FORC_043

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FORC43_RS27815 Protein ID WP_001159871.1
Coordinates 30093..30398 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FORC43_RS27810 Protein ID WP_000813634.1
Coordinates 29873..30091 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC43_RS27785 25648..26073 + 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
FORC43_RS27790 26070..26420 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
FORC43_RS27795 26451..27875 + 1425 Protein_40 IS66-like element ISEc23 family transposase -
FORC43_RS27800 28129..29262 - 1134 WP_000555401.1 IS110-like element ISEc45 family transposase -
FORC43_RS27805 29323..29511 + 189 Protein_42 IS66 family transposase -
FORC43_RS27810 29873..30091 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FORC43_RS27815 30093..30398 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FORC43_RS27820 30399..31208 + 810 WP_000016979.1 site-specific integrase -
FORC43_RS27825 31346..31621 - 276 WP_000239529.1 hypothetical protein -
FORC43_RS27830 31615..32259 - 645 WP_000633911.1 AAA family ATPase -
FORC43_RS27835 32488..33459 + 972 WP_001103689.1 hypothetical protein -
FORC43_RS27840 33464..33856 + 393 WP_000340832.1 plasmid partitioning/stability family protein -
FORC43_RS27845 33888..34715 - 828 Protein_50 translesion error-prone DNA polymerase V subunit UmuC -
FORC43_RS27850 34726..35199 - 474 Protein_51 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B agg3D / agg3C / agg3C / agg3C / agg3B / agg3A / aap/aspU / pic 1..77422 77422


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T81610 WP_001159871.1 NZ_CP023552:30093-30398 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T81610 NZ_CP023552:30093-30398 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT81610 WP_000813634.1 NZ_CP023552:29873-30091 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT81610 NZ_CP023552:29873-30091 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References