Detailed information of TA system TA081604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 80445..80970
• Replicon: plasmid pO104_H21
• Accession: NZ_CP023542
• Organism: Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A0F6YRT4
• Locus tag: CR208_RS24310
• Protein ID: WP_021293254.1
• Coordinates: 80445..80750 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: CR208_RS24315
• Protein ID: WP_000813639.1
• Coordinates: 80752..80970 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR208_RS24280	76439..76612	+	174	Protein_84	DUF4113 domain-containing protein	-
CR208_RS24285	76614..77030	-	417	WP_001278818.1	plasmid partitioning/stability family protein	-
CR208_RS24290	77023..78003	-	981	WP_000688510.1	plasmid segregation protein ParM	-
CR208_RS24295	78418..78726	-	309	WP_000030199.1	hypothetical protein	-
CR208_RS24300	78813..79457	-	645	WP_001144036.1	ParA family protein	-
CR208_RS24305	79638..80444	-	807	WP_000016966.1	site-specific integrase	-
CR208_RS24310	80445..80750	-	306	WP_021293254.1	type II toxin-antitoxin system toxin CcdB	Toxin
CR208_RS24315	80752..80970	-	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CR208_RS24320	81566..81826	+	261	WP_000343102.1	hypothetical protein	-
CR208_RS24325	81823..82413	+	591	WP_021293255.1	hypothetical protein	-
CR208_RS24330	82431..82778	-	348	WP_033817147.1	hypothetical protein	-
CR208_RS24335	82907..83242	+	336	WP_021292998.1	colicin transporter	-
CR208_RS24340	83249..83619	-	371	Protein_96	colicin	-
CR208_RS24345	83679..84410	-	732	WP_000850413.1	complement resistance protein TraT	-
CR208_RS24905	84687..84797	-	111	Protein_98	IS481 family transposase	-
CR208_RS25010	84801..85015	-	215	Protein_99	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..161452	161452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11634.45 Da
• Isoelectric Point: 7.2010

>T81604 WP_021293254.1 NZ_CP023542:c80750-80445 [Escherichia coli O104:H21 str. CFSAN002236]
MQFKVYTYKRESRYRLFVDVQSGIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T81604 NZ_CP023542:c80750-80445 [Escherichia coli O104:H21 str. CFSAN002236]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGGCATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT81604 WP_000813639.1 NZ_CP023542:c80970-80752 [Escherichia coli O104:H21 str. CFSAN002236]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT81604 NZ_CP023542:c80970-80752 [Escherichia coli O104:H21 str. CFSAN002236]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0F6YRT4

Antitoxin

• AlphaFold DB: A0A7U9DIR5