Detailed information of TA system TA081602

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4105588..4106386
• Replicon: chromosome
• Accession: NZ_CP023541
• Organism: Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CR208_RS20030
• Protein ID: WP_000854902.1
• Coordinates: 4106009..4106386 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A093DMQ4
• Locus tag: CR208_RS20025
• Protein ID: WP_001280952.1
• Coordinates: 4105588..4105962 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR208_RS19990	4102424..4102879	+	456	WP_000581507.1	hypothetical protein	-
CR208_RS19995	4102958..4103191	+	234	WP_001119729.1	DUF905 family protein	-
CR208_RS20005	4103291..4104109	+	819	WP_001234626.1	DUF945 domain-containing protein	-
CR208_RS20010	4104164..4104649	+	486	WP_000849590.1	antirestriction protein	-
CR208_RS20015	4104665..4105141	+	477	WP_001186725.1	RadC family protein	-
CR208_RS20020	4105204..4105425	+	222	WP_000692312.1	DUF987 domain-containing protein	-
CR208_RS20025	4105588..4105962	+	375	WP_001280952.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CR208_RS20030	4106009..4106386	+	378	WP_000854902.1	TA system toxin CbtA family protein	Toxin
CR208_RS20035	4106383..4106871	+	489	WP_000779175.1	hypothetical protein	-
CR208_RS20040	4106883..4107080	+	198	WP_000839249.1	DUF957 domain-containing protein	-
CR208_RS20045	4107165..4108007	+	843	WP_001280537.1	DUF4942 domain-containing protein	-
CR208_RS20050	4108350..4108886	-	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
CR208_RS20055	4108888..4110066	-	1179	WP_000094963.1	type II secretion system protein GspL	-
CR208_RS20060	4110063..4111040	-	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD	4070566..4117822	47256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-118)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14307.29 Da
• Isoelectric Point: 7.2922

>T81602 WP_000854902.1 NZ_CP023541:4106009-4106386 [Escherichia coli O104:H21 str. CFSAN002236]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
NACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T81602 NZ_CP023541:4106009-4106386 [Escherichia coli O104:H21 str. CFSAN002236]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AATGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13719.50 Da
• Isoelectric Point: 6.6249

>AT81602 WP_001280952.1 NZ_CP023541:4105588-4105962 [Escherichia coli O104:H21 str. CFSAN002236]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS

Nucleotide

• Length: 375 bp

>AT81602 NZ_CP023541:4105588-4105962 [Escherichia coli O104:H21 str. CFSAN002236]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A093DMQ4