Detailed information of TA system TA081601

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3948963..3949617
• Replicon: chromosome
• Accession: NZ_CP023541
• Organism: Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: CR208_RS19190
• Protein ID: WP_000244777.1
• Coordinates: 3948963..3949370 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: CR208_RS19195
• Protein ID: WP_000354049.1
• Coordinates: 3949351..3949617 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR208_RS19170	3944920..3946653	-	1734	WP_000813215.1	single-stranded-DNA-specific exonuclease RecJ	-
CR208_RS19175	3946659..3947369	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
CR208_RS19180	3947394..3948290	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
CR208_RS19185	3948402..3948923	+	522	WP_001055874.1	flavodoxin FldB	-
CR208_RS19190	3948963..3949370	-	408	WP_000244777.1	toxin CptA	Toxin
CR208_RS19195	3949351..3949617	-	267	WP_000354049.1	FAD assembly factor SdhE	Antitoxin
CR208_RS19200	3949860..3950840	+	981	WP_000886095.1	tRNA-modifying protein YgfZ	-
CR208_RS19205	3950917..3951576	-	660	WP_000250274.1	hemolysin III family protein	-
CR208_RS19210	3951740..3952051	-	312	WP_001182954.1	N(4)-acetylcytidine aminohydrolase	-
CR208_RS19215	3952096..3953529	+	1434	WP_001363803.1	6-phospho-beta-glucosidase BglA	-
CR208_RS19220	3953586..3954329	-	744	WP_000951951.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T81601 WP_000244777.1 NZ_CP023541:c3949370-3948963 [Escherichia coli O104:H21 str. CFSAN002236]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T81601 NZ_CP023541:c3949370-3948963 [Escherichia coli O104:H21 str. CFSAN002236]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10533.09 Da
• Isoelectric Point: 5.1599

>AT81601 WP_000354049.1 NZ_CP023541:c3949617-3949351 [Escherichia coli O104:H21 str. CFSAN002236]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFVRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT81601 NZ_CP023541:c3949617-3949351 [Escherichia coli O104:H21 str. CFSAN002236]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTGTTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7