81600

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3786469..3787052 Replicon chromosome
Accession NZ_CP023541
Organism Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag CR208_RS18430 Protein ID WP_000254739.1
Coordinates 3786469..3786804 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag CR208_RS18435 Protein ID WP_000581937.1
Coordinates 3786804..3787052 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR208_RS18415 3782356..3783654 - 1299 WP_000036723.1 phosphopyruvate hydratase -
CR208_RS18420 3783742..3785379 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
CR208_RS18425 3785607..3786398 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
CR208_RS18430 3786469..3786804 - 336 WP_000254739.1 endoribonuclease MazF Toxin
CR208_RS18435 3786804..3787052 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
CR208_RS18440 3787130..3789364 - 2235 WP_000226816.1 GTP diphosphokinase -
CR208_RS18445 3789412..3790713 - 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12126.09 Da        Isoelectric Point: 8.2618

>T81600 WP_000254739.1 NZ_CP023541:c3786804-3786469 [Escherichia coli O104:H21 str. CFSAN002236]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVVPEELQLIKAKINVLIG

Download         Length: 336 bp

>T81600 NZ_CP023541:c3786804-3786469 [Escherichia coli O104:H21 str. CFSAN002236]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGTCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT81600 WP_000581937.1 NZ_CP023541:c3787052-3786804 [Escherichia coli O104:H21 str. CFSAN002236]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT81600 NZ_CP023541:c3787052-3786804 [Escherichia coli O104:H21 str. CFSAN002236]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References