Detailed information of TA system TA081588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2307668..2308306
• Replicon: chromosome
• Accession: NZ_CP023541
• Organism: Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: CR208_RS11260
• Protein ID: WP_000813794.1
• Coordinates: 2307668..2307844 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CR208_RS11265
• Protein ID: WP_001270286.1
• Coordinates: 2307890..2308306 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR208_RS11240	2303287..2304501	-	1215	WP_071883264.1	BenE family transporter YdcO	-
CR208_RS11245	2304554..2305090	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
CR208_RS11250	2305163..2307124	+	1962	WP_021293044.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CR208_RS11255	2307216..2307446	-	231	WP_000494244.1	YncJ family protein	-
CR208_RS11260	2307668..2307844	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CR208_RS11265	2307890..2308306	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CR208_RS11270	2308385..2309791	+	1407	WP_021293381.1	PLP-dependent aminotransferase family protein	-
CR208_RS11275	2310036..2311181	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
CR208_RS11280	2311199..2312212	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
CR208_RS11285	2312213..2313154	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2301982..2303247	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T81588 WP_000813794.1 NZ_CP023541:2307668-2307844 [Escherichia coli O104:H21 str. CFSAN002236]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T81588 NZ_CP023541:2307668-2307844 [Escherichia coli O104:H21 str. CFSAN002236]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT81588 WP_001270286.1 NZ_CP023541:2307890-2308306 [Escherichia coli O104:H21 str. CFSAN002236]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT81588 NZ_CP023541:2307890-2308306 [Escherichia coli O104:H21 str. CFSAN002236]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB