Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 586667..587262 Replicon chromosome
Accession NZ_CP023541
Organism Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag CR208_RS02760 Protein ID WP_000239579.1
Coordinates 586912..587262 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag CR208_RS02755 Protein ID WP_001223208.1
Coordinates 586667..586918 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR208_RS02745 582332..586111 + 3780 WP_000060896.1 autotransporter assembly complex protein TamB -
CR208_RS02750 586114..586455 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
CR208_RS02755 586667..586918 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
CR208_RS02760 586912..587262 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
CR208_RS02765 587342..587872 - 531 WP_000055075.1 inorganic diphosphatase -
CR208_RS02770 588182..589138 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
CR208_RS02775 589278..590780 + 1503 WP_000210557.1 sugar ABC transporter ATP-binding protein -
CR208_RS02780 590794..591816 + 1023 WP_001297255.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T81583 WP_000239579.1 NZ_CP023541:586912-587262 [Escherichia coli O104:H21 str. CFSAN002236]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T81583 NZ_CP023541:586912-587262 [Escherichia coli O104:H21 str. CFSAN002236]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT81583 WP_001223208.1 NZ_CP023541:586667-586918 [Escherichia coli O104:H21 str. CFSAN002236]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT81583 NZ_CP023541:586667-586918 [Escherichia coli O104:H21 str. CFSAN002236]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References