Detailed information of TA system TA081574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 230481..231125
• Replicon: chromosome
• Accession: NZ_CP023536
• Organism: Providencia alcalifaciens strain FDAARGOS_408

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: B6XA97
• Locus tag: CO695_RS01165
• Protein ID: WP_004905417.1
• Coordinates: 230922..231125 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CO695_RS01160
• Protein ID: WP_006662760.1
• Coordinates: 230481..230849 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO695_RS01135	225680..226441	-	762	WP_006657237.1	DNA polymerase III subunit epsilon	-
CO695_RS01140	226496..226966	+	471	WP_006657236.1	ribonuclease HI	-
CO695_RS01145	226979..227704	-	726	WP_006657235.1	methyltransferase domain-containing protein	-
CO695_RS01150	227741..228493	+	753	WP_006657234.1	hydroxyacylglutathione hydrolase	-
CO695_RS01155	228576..229955	+	1380	WP_036961894.1	murein transglycosylase D	-
CO695_RS01160	230481..230849	+	369	WP_006662760.1	Hha toxicity modulator TomB	Antitoxin
CO695_RS01165	230922..231125	+	204	WP_004905417.1	hemolysin expression modulator Hha	Toxin
CO695_RS01175	231740..232198	-	459	WP_006662761.1	YbaY family lipoprotein	-
CO695_RS01180	232437..233303	+	867	WP_006657230.1	acyl-CoA thioesterase II	-
CO695_RS01185	233450..235231	-	1782	WP_006657229.1	SmdB family multidrug efflux ABC transporter permease/ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8061.34 Da
• Isoelectric Point: 6.9770

>T81574 WP_004905417.1 NZ_CP023536:230922-231125 [Providencia alcalifaciens]
MTKSDYLMRLRKCTTIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Nucleotide

• Length: 204 bp

>T81574 NZ_CP023536:230922-231125 [Providencia alcalifaciens]
ATGACCAAATCCGATTATCTGATGCGTTTAAGAAAATGCACCACTATTGAAACACTTGAGCGCGTTATTGAAAAAAATAA
GTATGAGTTATCCGATGATGAGTTAGAGTTATTTTACTCTGCCGCTGATCATCGTCTTGCAGAGCTCACGATGAATAAAC
TTTACGATAAAATTCCAGCATCTGTTTGGAAGTTTGTTAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14087.96 Da
• Isoelectric Point: 4.4687

>AT81574 WP_006662760.1 NZ_CP023536:230481-230849 [Providencia alcalifaciens]
MDEYSPKKHDIAELKYLCNSLNRDAISSLQKTNTHWINDLSSAQSISLNELVEHIAAFVWRFKIKYPKENLVISLVEEYL
DETYNLFGSPVVTFSEIIDWESMNQNLVAVLDDDLKCLTSKT

Nucleotide

• Length: 369 bp

>AT81574 NZ_CP023536:230481-230849 [Providencia alcalifaciens]
ATGGATGAATACTCACCCAAGAAACATGATATAGCCGAATTAAAATATCTTTGTAACAGTTTGAATAGAGACGCTATATC
AAGTTTACAAAAAACAAATACTCATTGGATAAATGATCTTAGTTCTGCACAAAGCATCAGTCTTAACGAGCTTGTTGAGC
ATATTGCAGCTTTTGTCTGGCGATTTAAAATCAAATATCCAAAAGAAAATCTCGTTATTTCTCTTGTTGAAGAGTATCTT
GACGAAACATATAATTTATTTGGCAGCCCAGTAGTTACCTTCAGCGAAATCATTGACTGGGAAAGTATGAATCAAAATCT
TGTCGCCGTGCTCGATGATGATCTAAAATGTCTGACAAGTAAGACTTAG

Structures

Toxin

• AlphaFold DB: A0A291E6Q2