Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5203..5728 Replicon plasmid unnamed2
Accession NZ_CP023533
Organism Escherichia coli strain FDAARGOS_403

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0E0Y871
Locus tag CO706_RS00155 Protein ID WP_001159872.1
Coordinates 5423..5728 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0E0Y6P9
Locus tag CO706_RS00150 Protein ID WP_000813638.1
Coordinates 5203..5421 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO706_RS00115 387..512 + 126 WP_032159539.1 hypothetical protein -
CO706_RS00120 633..1373 + 741 WP_001066951.1 tyrosine-type recombinase/integrase -
CO706_RS00125 1652..2629 - 978 WP_001387465.1 RepB family plasmid replication initiator protein -
CO706_RS00135 3715..3870 + 156 WP_000332512.1 transposase domain-containing protein -
CO706_RS00140 3953..4354 - 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin -
CO706_RS00145 4378..4608 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CO706_RS00150 5203..5421 + 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CO706_RS00155 5423..5728 + 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB Toxin
CO706_RS00160 5729..6535 + 807 WP_000016961.1 site-specific integrase -
CO706_RS00165 6714..7358 + 645 WP_001144032.1 ParA family protein -
CO706_RS00170 7445..7753 + 309 WP_000030204.1 molecular chaperone GroEL -
CO706_RS00175 8167..9147 + 981 WP_000688504.1 plasmid segregation protein ParM -
CO706_RS00180 9140..9556 + 417 WP_001278815.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aggD / aggC / aggB / aggA / aap/aspU 1..75566 75566


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11722.51 Da        Isoelectric Point: 5.6919

>T81545 WP_001159872.1 NZ_CP023533:5423-5728 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHWENDIKNAINLMFWGI

Download         Length: 306 bp

>T81545 NZ_CP023533:5423-5728 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTAATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACTGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8344.29 Da        Isoelectric Point: 4.5203

>AT81545 WP_000813638.1 NZ_CP023533:5203-5421 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVAKFIEMNGSFADENRDW

Download         Length: 219 bp

>AT81545 NZ_CP023533:5203-5421 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAAGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y871

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6P9

References