Detailed information of TA system TA081544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3953..4608
• Replicon: plasmid unnamed2
• Accession: NZ_CP023533
• Organism: Escherichia coli strain FDAARGOS_403

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A2H3M3N3
• Locus tag: CO706_RS00140
• Protein ID: WP_001392130.1
• Coordinates: 3953..4354 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A0E0YAG4
• Locus tag: CO706_RS00145
• Protein ID: WP_001261288.1
• Coordinates: 4378..4608 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO706_RS00110	103..264	+	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CO706_RS00115	387..512	+	126	WP_032159539.1	hypothetical protein	-
CO706_RS00120	633..1373	+	741	WP_001066951.1	tyrosine-type recombinase/integrase	-
CO706_RS00125	1652..2629	-	978	WP_001387465.1	RepB family plasmid replication initiator protein	-
CO706_RS00135	3715..3870	+	156	WP_000332512.1	transposase domain-containing protein	-
CO706_RS00140	3953..4354	-	402	WP_001392130.1	type II toxin-antitoxin system VapC family toxin	Toxin
CO706_RS00145	4378..4608	-	231	WP_001261288.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CO706_RS00150	5203..5421	+	219	WP_000813638.1	type II toxin-antitoxin system antitoxin CcdA	-
CO706_RS00155	5423..5728	+	306	WP_001159872.1	type II toxin-antitoxin system toxin CcdB	-
CO706_RS00160	5729..6535	+	807	WP_000016961.1	site-specific integrase	-
CO706_RS00165	6714..7358	+	645	WP_001144032.1	ParA family protein	-
CO706_RS00170	7445..7753	+	309	WP_000030204.1	molecular chaperone GroEL	-
CO706_RS00175	8167..9147	+	981	WP_000688504.1	plasmid segregation protein ParM	-
CO706_RS00180	9140..9556	+	417	WP_001278815.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	aggD / aggC / aggB / aggA / aap/aspU	1..75566	75566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14647.00 Da
• Isoelectric Point: 6.7123

>T81544 WP_001392130.1 NZ_CP023533:c4354-3953 [Escherichia coli]
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFDATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAETPIGPNNTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 402 bp

>T81544 NZ_CP023533:c4354-3953 [Escherichia coli]
ATGCTCGACACGAATATCTGCTCTTTCATTATGCGTGAACAGCCCGCCGCAGTGATCAAACGTCTGGAGCAGGCGGTGCT
GCGTAACCACCGCATTGTCGTGTCGGCCATCACCTATGCGGAGATGCGTTTCGACGCCACCGGCCCGAAAGCCTCACCGC
GTCATATTGCGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATTCTGCCCTGGGACCGCGCTGCCGTGGACGCCACC
ACGGAGATTAAAATGGCACTGCGGCTGGCCGAGACGCCAATCGGCCCGAACAACACGGCGATTGCCGGGCACGCCATCGC
TGCCGGGGCGATACTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGACCTGGTGCTGGAAGACTGGGTGAAGT
AA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8684.76 Da
• Isoelectric Point: 4.3654

>AT81544 WP_001261288.1 NZ_CP023533:c4608-4378 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT81544 NZ_CP023533:c4608-4378 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTTGAGATCGTCCGCGAAGGAGACAGCATTATATTGCGCCCGGTCCGGCCGACCTGGAGCTCGTTTGCGGCACTCG
AAAAGGCCGACGCAGACTTTATGGCGGAGCGTGAGGACATTGTCAGCGACGAAGGAAGATTTGACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A2H3M3N3

Antitoxin

• AlphaFold DB: A0A0E0YAG4