Detailed information of TA system TA081532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2109582..2110063
• Replicon: chromosome
• Accession: NZ_CP023531
• Organism: Escherichia coli strain FDAARGOS_401

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7YS45
• Locus tag: CO703_RS11715
• Protein ID: WP_014966210.1
• Coordinates: 2109773..2110063 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A0D7Y007
• Locus tag: CO703_RS11710
• Protein ID: WP_000100896.1
• Coordinates: 2109582..2109773 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO703_RS28840	2104734..2104912	-	179	Protein_2055	hypothetical protein	-
CO703_RS11670	2104914..2105663	-	750	WP_001289900.1	ead/Ea22-like family protein	-
CO703_RS11675	2105660..2106346	-	687	WP_000034251.1	ead/Ea22-like family protein	-
CO703_RS11680	2106333..2106827	-	495	WP_001275731.1	class I SAM-dependent methyltransferase	-
CO703_RS11685	2106824..2107246	-	423	WP_000416574.1	DUF977 family protein	-
CO703_RS11690	2107287..2108249	-	963	WP_000095671.1	helix-turn-helix domain-containing protein	-
CO703_RS11695	2108272..2108697	-	426	WP_000693845.1	Rha family transcriptional regulator	-
CO703_RS11700	2108681..2108956	-	276	WP_001048458.1	helix-turn-helix domain-containing protein	-
CO703_RS11705	2109064..2109564	+	501	WP_000824162.1	helix-turn-helix transcriptional regulator	-
CO703_RS11710	2109582..2109773	+	192	WP_000100896.1	hypothetical protein	Antitoxin
CO703_RS11715	2109773..2110063	+	291	WP_014966210.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CO703_RS11725	2110333..2110485	+	153	WP_000379548.1	DUF1391 family protein	-
CO703_RS11730	2110497..2110835	+	339	WP_000394541.1	hypothetical protein	-
CO703_RS11735	2110824..2111090	-	267	WP_012601410.1	hypothetical protein	-
CO703_RS11745	2111585..2111773	+	189	WP_000449175.1	cell division inhibitor	-
CO703_RS11750	2111770..2111958	+	189	WP_000199475.1	DUF1482 family protein	-
CO703_RS11755	2112051..2114489	+	2439	WP_000102130.1	exonuclease	-
CO703_RS11760	2114551..2114820	+	270	WP_000003742.1	excisionase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2062752..2123076	60324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11176.05 Da
• Isoelectric Point: 9.9854

>T81532 WP_014966210.1 NZ_CP023531:2109773-2110063 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLTEHPYLYPPSERVPSLREIVTHPNYIILYRVTASNIE
IVNVVHSRRQYPNKTC

Nucleotide

• Length: 291 bp

>T81532 NZ_CP023531:2109773-2110063 [Escherichia coli]
ATGCTGCCCATTTTATGGCTACCTTCTGCACGTGATGATTTACGTCAGATCGTAGCCTATATTGCTAAGGAAAACCCTCC
CGCTGCACGTAGACTAAAAATACGCATTGAAACATCAGTTTTGTCACTTACTGAACACCCTTATCTGTACCCACCGAGCG
AAAGAGTTCCAAGTCTTCGTGAGATAGTGACTCATCCTAACTACATAATACTTTACCGAGTAACAGCATCTAACATCGAG
ATCGTAAATGTAGTTCACTCACGAAGACAGTATCCAAACAAAACCTGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7033.83 Da
• Isoelectric Point: 4.0270

>AT81532 WP_000100896.1 NZ_CP023531:2109582-2109773 [Escherichia coli]
MSTALSPIISEFETVEQENSYNEWLRTKVAASLSDPRPAIPHDEVMAEMENLIAQLAATNRSE

Nucleotide

• Length: 192 bp

>AT81532 NZ_CP023531:2109582-2109773 [Escherichia coli]
ATGAGTACAGCCCTTTCCCCGATAATTTCTGAATTTGAAACAGTCGAACAAGAAAACAGCTATAACGAATGGTTGCGAAC
CAAAGTGGCAGCAAGCCTCTCAGATCCCCGTCCTGCAATTCCACATGACGAAGTAATGGCTGAAATGGAAAACCTTATTG
CTCAATTAGCTGCAACGAACAGGAGTGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0D7YS45

Antitoxin

• AlphaFold DB: A0A0D7Y007