Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 62896..63421 Replicon plasmid unnamed
Accession NZ_CP023530
Organism Escherichia coli strain FDAARGOS_401

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0E0Y871
Locus tag CO703_RS00420 Protein ID WP_001159872.1
Coordinates 63116..63421 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0E0Y6P9
Locus tag CO703_RS00415 Protein ID WP_000813638.1
Coordinates 62896..63114 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO703_RS00380 58080..58205 + 126 WP_032159539.1 hypothetical protein -
CO703_RS00385 58326..59066 + 741 WP_001066951.1 tyrosine-type recombinase/integrase -
CO703_RS00390 59345..60322 - 978 WP_001387465.1 RepB family plasmid replication initiator protein -
CO703_RS00400 61408..61563 + 156 WP_000332512.1 transposase domain-containing protein -
CO703_RS00405 61646..62047 - 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin -
CO703_RS00410 62071..62301 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CO703_RS00415 62896..63114 + 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CO703_RS00420 63116..63421 + 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB Toxin
CO703_RS00425 63422..64228 + 807 WP_000016961.1 site-specific integrase -
CO703_RS00430 64407..65051 + 645 WP_001144032.1 ParA family protein -
CO703_RS00435 65138..65446 + 309 WP_000030204.1 molecular chaperone GroEL -
CO703_RS00440 65860..66840 + 981 WP_000688504.1 plasmid segregation protein ParM -
CO703_RS00445 66833..67249 + 417 WP_001278815.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aap/aspU / aggD / aggC / aggB / aggA 1..75554 75554


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11722.51 Da        Isoelectric Point: 5.6919

>T81518 WP_001159872.1 NZ_CP023530:63116-63421 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHWENDIKNAINLMFWGI

Download         Length: 306 bp

>T81518 NZ_CP023530:63116-63421 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTAATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACTGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8344.29 Da        Isoelectric Point: 4.5203

>AT81518 WP_000813638.1 NZ_CP023530:62896-63114 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVAKFIEMNGSFADENRDW

Download         Length: 219 bp

>AT81518 NZ_CP023530:62896-63114 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAAGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y871

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6P9

References