Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 56784..57343 Replicon plasmid unnamed
Accession NZ_CP023530
Organism Escherichia coli strain FDAARGOS_401

Toxin (Protein)


Gene name relE Uniprot ID G4VUF8
Locus tag CO703_RS00365 Protein ID WP_000421273.1
Coordinates 57068..57343 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag CO703_RS00360 Protein ID WP_001387467.1
Coordinates 56784..57068 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO703_RS00305 51823..52500 - 678 WP_001339397.1 IS66 family insertion sequence hypothetical protein -
CO703_RS00310 52565..52777 + 213 Protein_57 ANR family transcriptional regulator -
CO703_RS00325 53228..54208 - 981 WP_000019427.1 IS5-like element ISKpn26 family transposase -
CO703_RS00330 54489..54743 + 255 WP_000083850.1 replication regulatory protein RepA -
CO703_RS29390 54850..54984 + 135 Protein_60 protein CopA/IncA -
CO703_RS00340 54981..55055 + 75 WP_014966221.1 RepA leader peptide Tap -
CO703_RS00345 55048..55876 + 829 Protein_62 incFII family plasmid replication initiator RepA -
CO703_RS00360 56784..57068 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
CO703_RS00365 57068..57343 + 276 WP_000421273.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CO703_RS00370 57349..57680 + 332 Protein_65 type II toxin-antitoxin system ParD family antitoxin -
CO703_RS00375 57796..57957 + 162 WP_001440647.1 type II toxin-antitoxin system RelE/ParE family toxin -
CO703_RS00380 58080..58205 + 126 WP_032159539.1 hypothetical protein -
CO703_RS00385 58326..59066 + 741 WP_001066951.1 tyrosine-type recombinase/integrase -
CO703_RS00390 59345..60322 - 978 WP_001387465.1 RepB family plasmid replication initiator protein -
CO703_RS00400 61408..61563 + 156 WP_000332512.1 transposase domain-containing protein -
CO703_RS00405 61646..62047 - 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin -
CO703_RS00410 62071..62301 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aap/aspU / aggD / aggC / aggB / aggA 1..75554 75554


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10765.45 Da        Isoelectric Point: 9.9324

>T81516 WP_000421273.1 NZ_CP023530:57068-57343 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T81516 NZ_CP023530:57068-57343 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT81516 WP_001387467.1 NZ_CP023530:56784-57068 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT81516 NZ_CP023530:56784-57068 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6H1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References