Detailed information of TA system TA081514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3402526..3403142
• Replicon: chromosome
• Accession: NZ_CP023529
• Organism: Lelliottia amnigena strain FDAARGOS_395

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3S5XV95
• Locus tag: CO697_RS16590
• Protein ID: WP_059180558.1
• Coordinates: 3402526..3402900 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CO697_RS16595
• Protein ID: WP_164841605.1
• Coordinates: 3402900..3403142 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO697_RS16575	3400006..3400929	+	924	WP_015961045.1	formate dehydrogenase subunit beta	-
CO697_RS16580	3400926..3401561	+	636	WP_059180560.1	formate dehydrogenase cytochrome b556 subunit	-
CO697_RS16585	3401558..3402487	+	930	WP_059180559.1	formate dehydrogenase accessory protein FdhE	-
CO697_RS16590	3402526..3402900	-	375	WP_059180558.1	type II toxin-antitoxin system VapC family toxin	Toxin
CO697_RS16595	3402900..3403142	-	243	WP_164841605.1	ribbon-helix-helix domain-containing protein	Antitoxin
CO697_RS16600	3403348..3404289	-	942	WP_041689564.1	fatty acid biosynthesis protein FabY	-
CO697_RS16605	3404334..3404771	-	438	WP_059180556.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CO697_RS16610	3404768..3405640	-	873	WP_059180555.1	virulence factor BrkB family protein	-
CO697_RS16615	3405634..3406233	-	600	WP_015961052.1	glucose-1-phosphatase	-
CO697_RS16620	3406366..3407166	-	801	WP_059180554.1	DeoR/GlpR family DNA-binding transcription regulator	-
CO697_RS16625	3407201..3408097	-	897	WP_059180553.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14003.14 Da
• Isoelectric Point: 7.4014

>T81514 WP_059180558.1 NZ_CP023529:c3402900-3402526 [Lelliottia amnigena]
MAKGSALFDTNILIDLFSGRAEAKHALESWPPQNAISIITWMEVMVGAKKYHQEHRTRIALSAFNVLGVSQEVAERSVDL
RQEYRMKLPDAIILATAQIHRLELVTRNTKDFGNIPDVVTPYNL

Nucleotide

• Length: 375 bp

>T81514 NZ_CP023529:c3402900-3402526 [Lelliottia amnigena]
ATGGCGAAAGGGTCTGCTCTTTTTGACACCAATATTCTCATCGACTTGTTCAGTGGAAGGGCAGAGGCAAAGCACGCATT
AGAGAGTTGGCCGCCGCAAAATGCGATTAGCATCATCACCTGGATGGAAGTCATGGTGGGAGCAAAAAAATACCATCAGG
AGCATCGTACCCGGATTGCCTTAAGTGCGTTTAACGTCCTTGGTGTTTCACAAGAGGTTGCTGAGCGGAGTGTTGATCTC
CGGCAGGAGTATCGAATGAAGCTCCCGGATGCCATTATTCTGGCTACTGCACAAATTCATCGTCTTGAACTGGTTACGCG
TAATACCAAGGATTTCGGTAACATCCCTGATGTGGTCACGCCCTACAATTTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9434.62 Da
• Isoelectric Point: 4.6034

>AT81514 WP_164841605.1 NZ_CP023529:c3403142-3402900 [Lelliottia amnigena]
MMAGRDMGRILLDLSDEVIKRLDDLKVHRNVPRAELLREAVEQYLERHDQAESTISDALGLWHGCEEEGVDYQRKLREEW

Nucleotide

• Length: 243 bp

>AT81514 NZ_CP023529:c3403142-3402900 [Lelliottia amnigena]
ATGATGGCGGGAAGGGACATGGGCAGAATTCTACTCGATCTATCAGACGAGGTGATAAAACGGCTGGACGACCTCAAGGT
GCACCGGAACGTCCCTCGCGCTGAATTGTTGCGGGAAGCCGTTGAGCAATATCTTGAAAGACACGATCAGGCTGAAAGCA
CGATCTCAGATGCCCTTGGCCTGTGGCATGGCTGTGAGGAAGAGGGTGTTGATTATCAGCGCAAGCTTCGTGAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A3S5XV95