Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 6840..7460 Replicon chromosome
Accession NZ_CP023529
Organism Lelliottia amnigena strain FDAARGOS_395

Toxin (Protein)


Gene name Hha Uniprot ID A0A2S5C408
Locus tag CO697_RS00045 Protein ID WP_012016344.1
Coordinates 6840..7058 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3S5XLQ2
Locus tag CO697_RS00050 Protein ID WP_059177928.1
Coordinates 7086..7460 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO697_RS00015 2846..3106 + 261 WP_012016339.1 type B 50S ribosomal protein L31 -
CO697_RS00020 3109..3249 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
CO697_RS00025 3246..3956 - 711 WP_059177924.1 GNAT family N-acetyltransferase -
CO697_RS00030 4056..5516 + 1461 WP_059177925.1 PLP-dependent aminotransferase family protein -
CO697_RS00035 5488..5955 - 468 WP_059177926.1 hypothetical protein -
CO697_RS00040 6074..6625 - 552 WP_059177927.1 maltose O-acetyltransferase -
CO697_RS00045 6840..7058 - 219 WP_012016344.1 hemolysin expression modulator Hha Toxin
CO697_RS00050 7086..7460 - 375 WP_059177928.1 Hha toxicity modulator TomB Antitoxin
CO697_RS00055 7969..11118 - 3150 WP_059177929.1 multidrug efflux RND transporter permease subunit AcrB -
CO697_RS00060 11141..12334 - 1194 WP_059177930.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8631.05 Da        Isoelectric Point: 8.9039

>T81501 WP_012016344.1 NZ_CP023529:c7058-6840 [Lelliottia amnigena]
MSDKPLTKTDYLMRLRRCQSLDTLERVIEKNKYELPDHELAVFYSAADHRLAELTMNKLYDKIPVSVWKFVR

Download         Length: 219 bp

>T81501 NZ_CP023529:c7058-6840 [Lelliottia amnigena]
ATGTCTGATAAACCATTAACTAAGACAGATTATTTGATGCGTTTGCGTCGCTGTCAGTCACTCGACACGCTTGAACGTGT
TATTGAAAAAAATAAATATGAGCTGCCCGATCATGAACTGGCTGTATTTTATTCAGCCGCAGATCATCGTCTTGCCGAGC
TAACCATGAATAAACTGTATGACAAAATCCCGGTGTCTGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14550.26 Da        Isoelectric Point: 5.1481

>AT81501 WP_059177928.1 NZ_CP023529:c7460-7086 [Lelliottia amnigena]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFNNVSRANPVSHSC

Download         Length: 375 bp

>AT81501 NZ_CP023529:c7460-7086 [Lelliottia amnigena]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTCGC
CAATCTTGATGAAAGCAACCATGGCTGGGTTAATGATCCGACCTCTGCAATCAACTTACAGCTTAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGATGAATATCTG
GACGACACCTTTATGTTGTTCAGTAGTTATGGCATTAACACGCAAGACTTGCAAAAATGGCGTAAATCAGGAAATCGGCT
ATTCCGTTGTTTCAACAACGTCAGCAGAGCTAATCCGGTAAGTCATTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S5C408

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5XLQ2

References