Detailed information of TA system TA081500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4802619..4803235
• Replicon: chromosome
• Accession: NZ_CP023527
• Organism: Citrobacter koseri strain FDAARGOS_393

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A427P7X6
• Locus tag: CO700_RS23505
• Protein ID: WP_047459878.1
• Coordinates: 4802861..4803235 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CO700_RS23500
• Protein ID: WP_160880417.1
• Coordinates: 4802619..4802861 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO700_RS23480	4799498..4800097	+	600	WP_058668370.1	glucose-1-phosphatase	-
CO700_RS23485	4800091..4800963	+	873	WP_012133924.1	virulence factor BrkB family protein	-
CO700_RS23490	4800960..4801397	+	438	WP_047459887.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CO700_RS23495	4801442..4802383	+	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
CO700_RS23500	4802619..4802861	+	243	WP_160880417.1	ribbon-helix-helix domain-containing protein	Antitoxin
CO700_RS23505	4802861..4803235	+	375	WP_047459878.1	type II toxin-antitoxin system VapC family toxin	Toxin
CO700_RS23510	4803272..4804201	-	930	WP_047459875.1	formate dehydrogenase accessory protein FdhE	-
CO700_RS23515	4804198..4804833	-	636	WP_058668371.1	formate dehydrogenase cytochrome b556 subunit	-
CO700_RS23520	4804830..4805732	-	903	WP_012133916.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13799.04 Da
• Isoelectric Point: 9.4395

>T81500 WP_047459878.1 NZ_CP023527:4802861-4803235 [Citrobacter koseri]
MIKGPVLFDTNILIDLFSGRDEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T81500 NZ_CP023527:4802861-4803235 [Citrobacter koseri]
ATGATTAAAGGTCCGGTGTTGTTTGACACCAATATTCTCATTGATTTATTTAGCGGGCGTGATGAAGCGAAGAAGGCTCT
CGAAGCTTACTCACCCCAGAATGCCATTAGTCTCATTACCTGGATGGAAGTGATGGTAGGGGCAAAAAAGTACCATCAGG
AATCCCGCACACGTATAGCATTGAGCGCTTTTAACGTTATCGGCGTTTCACAGGATATTGCCGAAAGAAGTGTCAGTCTC
AGGCAGGAATACGGGATGAAGTTGCCGGATGCGGTCATTCTGGCTACAGCGCACATTCATCGTTTTGCGCTCGTGACGCG
CAATACAAAGGACTTCGCGGGGATTCCGGGAGTCATTACGCCTTATCATTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9396.62 Da
• Isoelectric Point: 4.1961

>AT81500 WP_160880417.1 NZ_CP023527:4802619-4802861 [Citrobacter koseri]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW

Nucleotide

• Length: 243 bp

>AT81500 NZ_CP023527:4802619-4802861 [Citrobacter koseri]
ATGATGGCTGGGATGGAAATGGGCAGAATATTACTTGATTTATCAGATGAGGTTATCAAGCGGCTTGATGACCTTAAACA
ACAGCGTAATCTTCCGCGAGCAGAGCTATTACGCGAAGCAGTTGAACAATACCTGGAAAGGCAGGGACAAGCCGAAACCA
CTATTTCCGATGCGCTGGGGCTCTGGCAAGGTTGCGAAGAAGATGGCGTTGAATACGAAAGGAAACTTCGCGAGGAGTGG
TGA

Structures

Toxin

• AlphaFold DB: A0A427P7X6