Detailed information of TA system TA081487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1522429..1523041
• Replicon: chromosome
• Accession: NZ_CP023527
• Organism: Citrobacter koseri strain FDAARGOS_393

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: CO700_RS07375
• Protein ID: WP_012906750.1
• Coordinates: 1522862..1523041 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CO700_RS07370
• Protein ID: WP_047463070.1
• Coordinates: 1522429..1522839 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO700_RS07345	1517698..1518615	+	918	WP_060937433.1	baseplate J/gp47 family protein	-
CO700_RS07350	1518608..1519171	+	564	WP_058668939.1	phage tail protein I	-
CO700_RS07355	1519164..1520717	+	1554	WP_096753839.1	phage tail protein	-
CO700_RS07360	1520717..1521295	+	579	WP_096753840.1	tail fiber assembly protein	-
CO700_RS07365	1521388..1522371	-	984	WP_058667560.1	glycosyltransferase	-
CO700_RS07370	1522429..1522839	-	411	WP_047463070.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CO700_RS07375	1522862..1523041	-	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
CO700_RS07385	1523364..1523603	-	240	WP_058667562.1	hypothetical protein	-
CO700_RS07390	1523600..1523839	-	240	WP_058667564.1	DinI family protein	-
CO700_RS07395	1524165..1524488	+	324	WP_012133087.1	heavy metal-binding domain-containing protein	-
CO700_RS07400	1524485..1525315	+	831	WP_058668941.1	N-acetylmuramoyl-L-alanine amidase	-
CO700_RS07405	1525312..1526325	-	1014	WP_012133064.1	NAD(P)-dependent oxidoreductase	-
CO700_RS07410	1526424..1527854	-	1431	WP_058668942.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1482866..1525315	42449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T81487 WP_012906750.1 NZ_CP023527:c1523041-1522862 [Citrobacter koseri]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T81487 NZ_CP023527:c1523041-1522862 [Citrobacter koseri]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTAAAAGGCAGTCACCACCAGTT
CAAACACCCAACGAAGAAAGGGCTGGTAACGGTGAAACATCCACAAAAGGACATACCGCTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14887.79 Da
• Isoelectric Point: 4.3006

>AT81487 WP_047463070.1 NZ_CP023527:c1522839-1522429 [Citrobacter koseri]
MLYPIAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESVREAIDAHIELLVEDGEAVPEATSVENWLDDPDYAGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Nucleotide

• Length: 411 bp

>AT81487 NZ_CP023527:c1522839-1522429 [Citrobacter koseri]
ATGTTATATCCCATTGCTATTGATAAAGGCGATTCATCTTTCGGCGTTCGCGTACCGGATATTCCCGGCTGTTTTTCTGG
TGGTGATAACTACCAGGACGCAATCGAGAGTGTACGTGAAGCGATCGACGCACATATAGAATTACTGGTGGAAGATGGCG
AAGCAGTACCTGAAGCCACTAGTGTGGAAAACTGGCTGGACGATCCTGACTATGCTGGTGTGGTATGGGCGCTGGTGGAT
GTGGATGTTACGCGATTGATGGGGAAAGCTGAAAAAATCAATGTTACTCTTCCCTCTTTACTGATCCGACGTATCGACCA
GTTTGTCGCTGCACACCCTGAGTACGGTAGCCGCTCCGGCTTTCTGTCCCGTGTGGCGGCTGATAAGGTGATAGGACGAG
AAAAACGATAA

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4