Detailed information of TA system TA081461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 31890..33607
• Replicon: plasmid unnamed
• Accession: NZ_CP023524
• Organism: Burkholderia gladioli pv. gladioli strain FDAARGOS_389

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: CO712_RS35650
• Protein ID: WP_096753037.1
• Coordinates: 32963..33607 (-)
• Length: 215 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: CO712_RS35645
• Protein ID: WP_096753129.1
• Coordinates: 31890..32963 (-)
• Length: 358 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO712_RS37220	27596..28537	-	942	WP_138110708.1	hypothetical protein	-
CO712_RS35635	28541..30397	-	1857	WP_096753035.1	hypothetical protein	-
CO712_RS35640	30394..31296	-	903	WP_138110710.1	hypothetical protein	-
CO712_RS35645	31890..32963	-	1074	WP_096753129.1	macro domain-containing protein	Antitoxin
CO712_RS35650	32963..33607	-	645	WP_096753037.1	DUF4433 domain-containing protein	Toxin
CO712_RS35655	33916..35613	-	1698	WP_096753038.1	MCP four helix bundle domain-containing protein	-
CO712_RS35660	35706..36131	-	426	WP_096753039.1	hypothetical protein	-
CO712_RS35670	37561..37803	-	243	Protein_38	transposase	-
CO712_RS35675	37812..37994	-	183	Protein_39	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..151925	151925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (15-214)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 215 a.a.
• Molecular weight: 24799.62 Da
• Isoelectric Point: 9.0011

>T81461 WP_096753037.1 NZ_CP023524:c33607-32963 [Burkholderia gladioli pv. gladioli]
MATYQRLNAQNAYIWRIVHRQNLPWILDHGLVCASSGLLDPNFVPIGNADLISRRRLRAVPVAPGGTLSDYVPFYFTPFS
PMMYNIHTGRAVRQQRNEDICMLVSRLPVLQQMGIPFVFTDRHAYTQLARFFRDLGELGHIDWPLLQARNFKRNPDDPEQ
IERYQAEALVYHHLPVNGLTAIVCYTEQVKVELEQLVAARRLSVTVRALPHWYF

Nucleotide

• Length: 645 bp

>T81461 NZ_CP023524:c33607-32963 [Burkholderia gladioli pv. gladioli]
ATGGCGACATACCAGCGGCTCAACGCCCAGAACGCGTACATTTGGCGCATCGTTCATCGCCAGAATCTGCCGTGGATTCT
GGATCACGGGCTGGTGTGCGCGAGCAGCGGACTCCTCGACCCTAATTTCGTGCCGATCGGCAATGCGGATCTGATCAGCC
GGCGTAGGCTCCGAGCTGTGCCCGTCGCGCCTGGCGGAACGCTGAGTGACTACGTGCCATTTTATTTCACGCCGTTTTCG
CCAATGATGTACAACATTCATACTGGGCGGGCCGTGCGCCAGCAGCGCAACGAGGACATCTGCATGCTCGTCAGCAGGTT
GCCGGTCCTTCAGCAAATGGGCATACCGTTTGTCTTTACCGATCGACATGCATACACGCAGTTGGCGCGGTTCTTTCGCG
ATCTGGGGGAGTTGGGCCATATCGACTGGCCATTACTTCAGGCGCGGAACTTTAAGCGCAACCCGGACGATCCCGAGCAA
ATCGAACGCTACCAAGCTGAGGCACTGGTCTATCATCATCTGCCGGTGAACGGACTCACTGCGATTGTGTGCTATACCGA
ACAGGTGAAAGTTGAACTGGAGCAGTTGGTGGCCGCGCGTCGTTTGAGTGTGACGGTGCGAGCGCTCCCCCACTGGTACT
TTTGA

Antitoxin

Protein

• Length: 358 a.a.
• Molecular weight: 40369.40 Da
• Isoelectric Point: 6.1183

>AT81461 WP_096753129.1 NZ_CP023524:c32963-31890 [Burkholderia gladioli pv. gladioli]
MITYTQGNLLDAPAEALVNTVNTVGVMGKGIALMFKEKFPENFRQYALACKEGRVHTGTVFVTERMELIGPKWIVNFPTK
RHWRAPSRLEWIVDGLADLRHFIEKEHIRSIAIPPLGAGNGGLDWPTVKAEIDAALGDMVDTEILVYEPTGRYQNVSKRE
GVEKLTPARALIAELVRRYWILGMECTLLEIQKLAWFLERTIERTDAPNPLELKFVAHTYGPYANRLDHLLNNLDGSYLH
CDKRIADAGPLDVIWFDDRRVDMLAAYLKSGEMRPYLPALEETASLIDGFESPFGLELLASLDWLLARDGRDPNLDSIRA
GFSAWPGGESAGRRKYRLFDERAIQIGLRRLTEHVPA

Nucleotide

• Length: 1074 bp

>AT81461 NZ_CP023524:c32963-31890 [Burkholderia gladioli pv. gladioli]
ATGATTACATATACCCAAGGCAACCTGCTCGACGCTCCTGCCGAGGCGCTCGTCAACACGGTCAACACGGTTGGCGTGAT
GGGCAAAGGCATCGCGCTGATGTTTAAAGAAAAGTTTCCTGAGAACTTCCGCCAGTACGCGCTTGCCTGCAAAGAAGGGC
GCGTGCACACCGGCACGGTGTTCGTGACGGAACGCATGGAACTGATTGGGCCAAAGTGGATCGTCAACTTTCCGACTAAG
CGCCATTGGCGTGCGCCTTCACGGCTTGAGTGGATCGTCGACGGCCTAGCCGATCTACGCCACTTCATCGAAAAAGAACA
CATCCGATCGATCGCTATACCGCCGCTCGGCGCGGGCAATGGGGGTTTGGACTGGCCTACCGTGAAGGCCGAAATCGACG
CGGCGCTCGGCGATATGGTGGACACGGAGATTTTGGTCTACGAACCAACTGGCAGGTATCAGAATGTCTCAAAACGTGAG
GGTGTAGAGAAGCTGACCCCCGCCCGTGCATTGATCGCCGAGCTTGTGCGACGATACTGGATTCTTGGCATGGAATGCAC
TTTGCTTGAGATTCAGAAGCTCGCGTGGTTCCTCGAGCGCACGATCGAGCGGACCGATGCGCCAAACCCGCTCGAGCTCA
AGTTTGTCGCACATACCTATGGGCCGTATGCGAATCGCCTAGATCATCTGTTGAACAATCTTGATGGCAGTTATTTGCAC
TGCGACAAGCGCATCGCCGACGCTGGCCCACTCGATGTCATTTGGTTCGACGACCGTCGAGTCGACATGCTTGCGGCGTA
CCTGAAGAGCGGCGAGATGAGGCCGTATTTGCCCGCTCTTGAGGAGACCGCCTCTTTGATCGACGGATTTGAATCACCGT
TCGGGCTTGAGCTTCTCGCGTCGTTAGATTGGTTGTTGGCCCGCGACGGGCGCGATCCCAATCTCGATAGCATTCGTGCG
GGCTTCTCGGCGTGGCCGGGTGGCGAGTCGGCGGGGCGGCGGAAATATCGACTGTTCGACGAGAGGGCAATTCAGATTGG
TTTGAGACGCCTGACCGAGCACGTCCCTGCATGA