Detailed information of TA system TA081452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1714855..1715496
• Replicon: chromosome
• Accession: NZ_CP023521
• Organism: Burkholderia cepacia strain FDAARGOS_388

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CO711_RS32500
• Protein ID: WP_027790991.1
• Coordinates: 1714855..1715193 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: CO711_RS32505
• Protein ID: WP_021157290.1
• Coordinates: 1715197..1715496 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO711_RS32485	1711412..1712845	-	1434	WP_027790993.1	aldehyde dehydrogenase family protein	-
CO711_RS32490	1712875..1714515	-	1641	WP_027790992.1	acetolactate synthase large subunit	-
CO711_RS32500	1714855..1715193	+	339	WP_027790991.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CO711_RS32505	1715197..1715496	+	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
CO711_RS32510	1715553..1716839	-	1287	WP_027790990.1	DUF445 domain-containing protein	-
CO711_RS32515	1717051..1718271	-	1221	WP_027790989.1	FAD-dependent oxidoreductase	-
CO711_RS32520	1718261..1718587	-	327	WP_006479527.1	non-heme iron oxygenase ferredoxin subunit	-
CO711_RS32525	1718608..1719093	-	486	WP_034196026.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
CO711_RS32530	1719111..1720382	-	1272	WP_027790987.1	Rieske 2Fe-2S domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (16-109)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12591.43 Da
• Isoelectric Point: 9.9308

>T81452 WP_027790991.1 NZ_CP023521:1714855-1715193 [Burkholderia cepacia]
MVDIPQSMPYSAPTFSIRTTDVFDAWFAGLRDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLGGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T81452 NZ_CP023521:1714855-1715193 [Burkholderia cepacia]
ATGGTTGACATACCGCAATCAATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCATGGTT
TGCCGGCCTGCGCGATCGCGTCGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCGGGCTCGCCGGTCGTCGAAATGCGGATCGACCACGGCCCCGGCTATCGCGTCTACTACGTCCGGCGCGGA
ACGATCTGGGTCATCCTGCTCGGCGGCGGCGATAAATCGACGCAACAGGCCGACATACGCGCCGCGCACGCGATGCTCGC
GCACCTCGACATGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT81452 WP_021157290.1 NZ_CP023521:1715197-1715496 [Burkholderia cepacia]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT81452 NZ_CP023521:1715197-1715496 [Burkholderia cepacia]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAGACCGAAGACGACATGGCCGACTACTTCGAAGC
GTGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCACGCGCACGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCGAGCTTCGGCACC
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCATGCATGA

Structures

Antitoxin

• AlphaFold DB: U2GAF3