Detailed information of TA system TA081451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1324509..1325086
• Replicon: chromosome
• Accession: NZ_CP023521
• Organism: Burkholderia cepacia strain FDAARGOS_388

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U2G063
• Locus tag: CO711_RS30680
• Protein ID: WP_021160897.1
• Coordinates: 1324721..1325086 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2HEK9
• Locus tag: CO711_RS30675
• Protein ID: WP_021160898.1
• Coordinates: 1324509..1324727 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO711_RS30650	1319771..1320253	+	483	WP_027791292.1	GNAT family N-acetyltransferase	-
CO711_RS30655	1320341..1321606	-	1266	WP_027791291.1	Zn-dependent hydrolase	-
CO711_RS30660	1321599..1322123	-	525	WP_021160901.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
CO711_RS30665	1322178..1323344	-	1167	WP_027791290.1	branched-chain amino acid ABC transporter substrate-binding protein	-
CO711_RS30670	1323522..1324418	+	897	WP_027791289.1	LysR family transcriptional regulator	-
CO711_RS30675	1324509..1324727	+	219	WP_021160898.1	hypothetical protein	Antitoxin
CO711_RS30680	1324721..1325086	+	366	WP_021160897.1	type II toxin-antitoxin system VapC family toxin	Toxin
CO711_RS30685	1325425..1325988	+	564	WP_006488685.1	peroxiredoxin	-
CO711_RS30690	1326114..1327712	+	1599	WP_027791288.1	alkyl hydroperoxide reductase subunit F	-
CO711_RS41290	1327817..1328431	+	615	WP_155253146.1	hypothetical protein	-
CO711_RS30700	1328753..1329307	-	555	WP_027791286.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13448.50 Da
• Isoelectric Point: 8.4389

>T81451 WP_021160897.1 NZ_CP023521:1324721-1325086 [Burkholderia cepacia]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISTISWMEVLVGTSAQDEAPIRAWLSSFDVIALDSPVANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGVRVPYKL

Nucleotide

• Length: 366 bp

>T81451 NZ_CP023521:1324721-1325086 [Burkholderia cepacia]
ATGGTAAAAGCTCTCTTCGATACCAACATCCTGATCGACTATCTGGGCGGCGTGGAATCCGCTCGTGCGGAACTGGGCCG
CTACGACTATCGGGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGTCGGCACGTCCGCACAGGACGAGGCACCCATCC
GCGCGTGGCTGTCGTCGTTCGATGTCATCGCACTGGACAGCCCGGTTGCGAATCGTGCCGTGACAATCCGCAAGGAACGC
CGCATACGCCTTCCCGATGCAATCGTCTGGGCATCGGCACAAGTGAACGGTCTACTGCTCGTGTCGCGCAACACGAAGGA
CTTCCCTGCCACCGAACCCGGCGTTCGCGTGCCCTACAAGCTCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.51 Da
• Isoelectric Point: 5.0604

>AT81451 WP_021160898.1 NZ_CP023521:1324509-1324727 [Burkholderia cepacia]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALHKHRLADDVFGLWKDRKVDGLTYQEELRSEW

Nucleotide

• Length: 219 bp

>AT81451 NZ_CP023521:1324509-1324727 [Burkholderia cepacia]
ATGAGCCGAATTCTGGTCGATCTGTCGGACGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACATCGTCCCCG
CGCCGCAATCATCCGCGACGCGATCGATGCCTATATTGCCTTGCACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTCT
GGAAAGATCGCAAGGTCGACGGGCTCACCTATCAGGAAGAACTGCGCTCGGAATGGTAA

Structures

Toxin

• AlphaFold DB: U2G063

Antitoxin

• AlphaFold DB: U2HEK9