Detailed information of TA system TA081441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 251246..251820
• Replicon: chromosome
• Accession: NZ_CP023516
• Organism: Pasteurella multocida strain FDAARGOS_384

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: CO688_RS01325
• Protein ID: WP_096743047.1
• Coordinates: 251536..251820 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CO688_RS01320
• Protein ID: WP_096743046.1
• Coordinates: 251246..251539 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO688_RS01300	246420..247511	-	1092	WP_005751571.1	L-ascorbate 6-phosphate lactonase	-
CO688_RS01305	247861..249651	+	1791	WP_005726754.1	PTS ascorbate-specific subunit IIBC	-
CO688_RS01310	249696..250163	+	468	WP_005726755.1	PTS sugar transporter subunit IIA	-
CO688_RS01315	250182..250859	+	678	WP_096743045.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-
CO688_RS01320	251246..251539	-	294	WP_096743046.1	putative addiction module antidote protein	Antitoxin
CO688_RS01325	251536..251820	-	285	WP_096743047.1	hypothetical protein	Toxin
CO688_RS01335	253402..256563	+	3162	WP_138243246.1	STY4851/ECs_5259 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-87)

Antitoxin

• Uncharacterized protein HI1420 (7-92)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10642.30 Da
• Isoelectric Point: 10.1243

>T81441 WP_096743047.1 NZ_CP023516:c251820-251536 [Pasteurella multocida]
VGRLKEVKILSAKSAVLSRINRAMNGNFGDHKSIGNGLYEMRIIKGSGYRVYYGQYGEVTYLLICSGDKSTQKSDIVKAR
ELWKEIKQQEEVKV

Nucleotide

• Length: 285 bp

>T81441 NZ_CP023516:c251820-251536 [Pasteurella multocida]
GTGGGTCGGTTAAAGGAGGTAAAAATTTTATCCGCCAAATCTGCTGTTCTTTCTCGTATTAACAGAGCAATGAATGGTAA
TTTTGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATGGAGAAGTAACTTATTTATTAATTTGTAGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGCTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10982.83 Da
• Isoelectric Point: 8.0722

>AT81441 WP_096743046.1 NZ_CP023516:c251539-251246 [Pasteurella multocida]
MSKKKIELKPFDIAEHLDSEEMIAAYLSEVLETCDTNEFIFALGHVVRARGMTELAAKTGLRRESLYKTLSYGSKPRFDT
IMKITHSLGIKLVPTHI

Nucleotide

• Length: 294 bp

>AT81441 NZ_CP023516:c251539-251246 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAGAATTAAAACCATTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTTCGCTTTAGGTCATGTTGTAAGAGCAAGAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA