Detailed information of TA system TA081430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4155031..4155697
• Replicon: chromosome
• Accession: NZ_CP023504
• Organism: Citrobacter werkmanii strain FDAARGOS_364

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A6N6K5Y9
• Locus tag: CO701_RS20590
• Protein ID: WP_005132818.1
• Coordinates: 4155338..4155697 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: CO701_RS20585
• Protein ID: WP_096758754.1
• Coordinates: 4155031..4155348 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO701_RS20545	4150640..4151398	+	759	WP_096758746.1	DeoR/GlpR family DNA-binding transcription regulator	-
CO701_RS20550	4151472..4151744	+	273	WP_096758747.1	DUF3811 domain-containing protein	-
CO701_RS20555	4151741..4152616	-	876	WP_096758748.1	23S rRNA pseudouridine(2604) synthase RluF	-
CO701_RS20560	4152867..4153184	-	318	WP_096758749.1	CcdB family protein	-
CO701_RS20565	4153184..4153477	-	294	WP_096758750.1	type II toxin-antitoxin system CcdA family antitoxin	-
CO701_RS20570	4153574..4153939	-	366	WP_096758751.1	hypothetical protein	-
CO701_RS24615	4154071..4154244	+	174	WP_096758752.1	hypothetical protein	-
CO701_RS20580	4154743..4155021	+	279	WP_096758753.1	putative addiction module antidote protein	-
CO701_RS20585	4155031..4155348	-	318	WP_096758754.1	helix-turn-helix domain-containing protein	Antitoxin
CO701_RS20590	4155338..4155697	-	360	WP_005132818.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CO701_RS20595	4155911..4156600	+	690	WP_096758755.1	dipeptidase PepE	-
CO701_RS20600	4156729..4158360	-	1632	WP_096758756.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-87)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13476.49 Da
• Isoelectric Point: 10.3799

>T81430 WP_005132818.1 NZ_CP023504:c4155697-4155338 [Citrobacter werkmanii]
MTKPLYWVGHARKDLQGMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNTWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEINLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T81430 NZ_CP023504:c4155697-4155338 [Citrobacter werkmanii]
ATGACGAAACCACTTTACTGGGTTGGGCACGCCAGGAAAGACCTGCAGGGGATGCCAGAACATGTTCGGGATACGTTTGG
CTTTGCTCTTTGGCTGGCGCAGCAGGGTAAGCAGCATTCGCAGACTAAACCGTTAAAAGGATTTGGTGGGGCAGGGGTTC
TGGAAGTTGTTGAGGATTATCACGGCAACACATGGCGGGCGGTTTACACTATCCAGTTGAAAAATGCAGTTTATGTTCTA
CATGTTTTTCAGAAAAAATCTGTTTCCGGGAAGGCAACGCCAAAACCCGAAATTAACCTGATTTACCAGCGGCTTAAGGC
TGCACAACGGCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11301.08 Da
• Isoelectric Point: 4.7814

>AT81430 WP_096758754.1 NZ_CP023504:c4155348-4155031 [Citrobacter werkmanii]
MSHDVETSSGNVYADLGEYDAEQMQLKAQLAMVIGEILQCKGVTQQQAAEMLGMTQPKLSNLLRGQFRGISEAKMLECLV
KLGRDVAIVVGKEGDVPGTLKVSFG

Nucleotide

• Length: 318 bp

>AT81430 NZ_CP023504:c4155348-4155031 [Citrobacter werkmanii]
ATGTCACATGATGTCGAAACCAGTAGCGGCAATGTTTACGCCGATTTGGGTGAGTATGACGCAGAACAGATGCAGTTGAA
GGCGCAACTGGCAATGGTGATTGGCGAGATTTTGCAATGCAAAGGGGTAACACAGCAGCAGGCTGCAGAGATGCTGGGGA
TGACGCAGCCCAAGCTTTCCAACTTATTACGCGGGCAGTTTCGTGGCATCAGTGAAGCAAAGATGCTGGAGTGTCTGGTG
AAACTGGGTCGCGATGTGGCGATTGTGGTAGGAAAAGAGGGTGATGTGCCGGGCACGCTGAAAGTAAGCTTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A6N6K5Y9