Detailed information of TA system TA081426

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3803372..3804051
• Replicon: chromosome
• Accession: NZ_CP023504
• Organism: Citrobacter werkmanii strain FDAARGOS_364

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: CO701_RS18845
• Protein ID: WP_096758456.1
• Coordinates: 3803372..3803713 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: CO701_RS18850
• Protein ID: WP_096758457.1
• Coordinates: 3803734..3804051 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO701_RS18815	3798764..3799090	-	327	WP_003830102.1	DUF1493 family protein	-
CO701_RS18820	3799084..3799532	-	449	Protein_3568	hypothetical protein	-
CO701_RS18830	3800309..3801319	-	1011	WP_096758455.1	Ldh family oxidoreductase	-
CO701_RS18835	3801369..3801929	+	561	Protein_3570	HNH endonuclease	-
CO701_RS18840	3802111..3803118	-	1008	WP_057059518.1	restriction endonuclease	-
CO701_RS18845	3803372..3803713	-	342	WP_096758456.1	TA system toxin CbtA family protein	Toxin
CO701_RS18850	3803734..3804051	-	318	WP_096758457.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CO701_RS18855	3804070..3804291	-	222	WP_096758458.1	DUF987 family protein	-
CO701_RS18860	3804301..3804777	-	477	WP_096758459.1	RadC family protein	-
CO701_RS18865	3804790..3805248	-	459	WP_096758460.1	antirestriction protein	-
CO701_RS18870	3805344..3805583	-	240	WP_096758461.1	DUF905 domain-containing protein	-
CO701_RS18875	3805661..3806071	-	411	WP_096758462.1	hypothetical protein	-
CO701_RS18880	3806161..3806775	-	615	WP_096758463.1	hypothetical protein	-
CO701_RS18885	3806772..3807476	-	705	WP_096758464.1	WYL domain-containing protein	-
CO701_RS18890	3807678..3808511	-	834	WP_096758465.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12877.87 Da
• Isoelectric Point: 8.9922

>T81426 WP_096758456.1 NZ_CP023504:c3803713-3803372 [Citrobacter werkmanii]
MKTLPATTPQAAKLCLSPVSVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLADAVNFLVDKYELVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRINATL

Nucleotide

• Length: 342 bp

>T81426 NZ_CP023504:c3803713-3803372 [Citrobacter werkmanii]
ATGAAAACTCTACCTGCAACGACTCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGTCTGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGCCTGACACTGAACGACACACCATTCAGTGATGAGACTGTTATTCAGGAACATATCA
ATGCTGGTATTACACTGGCTGATGCCGTTAACTTTCTGGTGGATAAGTACGAACTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATACCTGCGGGCTGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAACGCAACACTTTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11835.41 Da
• Isoelectric Point: 6.4753

>AT81426 WP_096758457.1 NZ_CP023504:c3804051-3803734 [Citrobacter werkmanii]
MSNITWGLQRDITPHLGARLVQEGNRLHYLADRASITGKFSDAECLKLDEAFPHFISQMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYIYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT81426 NZ_CP023504:c3804051-3803734 [Citrobacter werkmanii]
ATGAGCAATATCACATGGGGCCTGCAGCGGGATATCACACCGCACCTGGGAGCCCGTCTGGTACAGGAGGGTAACCGGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGCTGAATGCTTGAAGCTGGATGAGGCATTTCCGC
ATTTTATCAGCCAGATGGAATCGATGTTGACCACTGGTGAAATGAATCCTCGCCACGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGTGAAGCCGATACCCTTGGCAGTTGCGGCTACATATACATCGCCGTTTATCCCACTCAACGCTAA