Detailed information of TA system TA081425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3265506..3266126
• Replicon: chromosome
• Accession: NZ_CP023504
• Organism: Citrobacter werkmanii strain FDAARGOS_364

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: CO701_RS16410
• Protein ID: WP_002892050.1
• Coordinates: 3265908..3266126 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CO701_RS16405
• Protein ID: WP_096758040.1
• Coordinates: 3265506..3265880 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO701_RS16395	3260652..3261845	+	1194	WP_096758038.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
CO701_RS16400	3261868..3265017	+	3150	WP_096758039.1	efflux RND transporter permease AcrB	-
CO701_RS16405	3265506..3265880	+	375	WP_096758040.1	Hha toxicity modulator TomB	Antitoxin
CO701_RS16410	3265908..3266126	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
CO701_RS16415	3266399..3266872	+	474	WP_096758041.1	YlaC family protein	-
CO701_RS16420	3266946..3267086	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
CO701_RS16425	3267088..3267348	-	261	WP_096758042.1	type B 50S ribosomal protein L31	-
CO701_RS16430	3267533..3269086	+	1554	WP_096758043.1	EAL domain-containing protein	-
CO701_RS16435	3269140..3269493	-	354	WP_096758044.1	DUF1428 family protein	-
CO701_RS16440	3269576..3270187	-	612	WP_096758045.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T81425 WP_002892050.1 NZ_CP023504:3265908-3266126 [Citrobacter werkmanii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T81425 NZ_CP023504:3265908-3266126 [Citrobacter werkmanii]
ATGTCTGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGCTGCCAGACAATTGACACGCTTGAGCGTGT
TATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACGATGAATAAGCTGTACGACAAGATTCCAACTTCCGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14427.23 Da
• Isoelectric Point: 5.5653

>AT81425 WP_096758040.1 NZ_CP023504:3265506-3265880 [Citrobacter werkmanii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINTQDLQKWRKSGSRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT81425 NZ_CP023504:3265506-3265880 [Citrobacter werkmanii]
ATGGACGAGTACTCACCCAAACGGCATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCTTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGCAACCATGGCTGGGTTAATGACCCGACTTCAGCAACCAGCCTGCAGCTCAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATTGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAATTATGGTATTAATACGCAGGATTTGCAGAAATGGCGAAAGTCCGGAAGTCGACT
GTTTCGCTGTTTTGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2