Detailed information of TA system TA081424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3151595..3152334
• Replicon: chromosome
• Accession: NZ_CP023504
• Organism: Citrobacter werkmanii strain FDAARGOS_364

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: CO701_RS15885
• Protein ID: WP_096757948.1
• Coordinates: 3151595..3152080 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: CO701_RS15890
• Protein ID: WP_096757949.1
• Coordinates: 3152068..3152334 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO701_RS15855	3147110..3147763	+	654	WP_096757942.1	enterobactin synthase subunit EntD	-
CO701_RS15860	3147753..3148832	-	1080	WP_096757943.1	alpha/beta hydrolase	-
CO701_RS15865	3148916..3149620	-	705	WP_096757944.1	GNAT family N-acetyltransferase	-
CO701_RS15875	3149888..3151006	+	1119	WP_096757946.1	glutamate--cysteine ligase	-
CO701_RS15880	3151073..3151321	+	249	WP_096757947.1	DUF1158 family protein	-
CO701_RS15885	3151595..3152080	-	486	WP_096757948.1	GNAT family N-acetyltransferase	Toxin
CO701_RS15890	3152068..3152334	-	267	WP_096757949.1	DUF1778 domain-containing protein	Antitoxin
CO701_RS15895	3152490..3152831	-	342	WP_096757950.1	RamA family antibiotic efflux transcriptional regulator	-
CO701_RS15900	3152848..3153960	-	1113	WP_096757951.1	MBL fold metallo-hydrolase	-
CO701_RS15905	3154150..3154731	+	582	WP_096757952.1	TetR/AcrR family transcriptional regulator	-
CO701_RS15910	3154731..3155099	+	369	WP_096757953.1	MmcQ/YjbR family DNA-binding protein	-
CO701_RS15915	3155219..3155872	+	654	WP_096757954.1	oxygen-insensitive NAD(P)H nitroreductase	-
CO701_RS15920	3155937..3157181	+	1245	WP_096757955.1	mechanosensitive ion channel family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17732.53 Da
• Isoelectric Point: 10.0703

>T81424 WP_096757948.1 NZ_CP023504:c3152080-3151595 [Citrobacter werkmanii]
VGRVTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQIAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVELSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T81424 NZ_CP023504:c3152080-3151595 [Citrobacter werkmanii]
GTGGGACGTGTAACAGCACCCGAACCTTTGTCCGCTTTTCATCAGGTGGCTGAGTTCGTCAGCGGTGAGGCTGTGCTCGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCACTCGGAGCGGCCAGAACGTTTGTGGTGTGTAAGAAAGACACGA
AGCAAATAGCCGGCTTTTACTCTCTGGCCACCGGGAGCGTCAATCATACTGAAGCAACCGGTAATCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGAACTCTCATTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCGTCACAAACTCAGCAGCGGACATTGTTCCTTAAGCTCCCT
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10104.52 Da
• Isoelectric Point: 4.3952

>AT81424 WP_096757949.1 NZ_CP023504:c3152334-3152068 [Citrobacter werkmanii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILERRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT81424 NZ_CP023504:c3152334-3152068 [Citrobacter werkmanii]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGATGCGGCTGCGGAAATCCTTCA
CAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCCTCGAGCGCCGTGTATTTAACT
TTAACGATGAGCAATATGCGGAGTTCATCGATATGCTTGATGCACCGGTCGCAGATGATCCCGCTATCGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA