Detailed information of TA system TA081404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1829751..1830280
• Replicon: chromosome
• Accession: NZ_CP023500
• Organism: Staphylococcus aureus strain FDAARGOS_412

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CO684_RS09660
• Protein ID: WP_000621175.1
• Coordinates: 1829751..1830113 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CO684_RS09665
• Protein ID: WP_000948331.1
• Coordinates: 1830110..1830280 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO684_RS09635	1826728..1827498	-	771	WP_096753292.1	RNA polymerase sigma factor SigB	-
CO684_RS09640	1827473..1827952	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
CO684_RS09645	1827954..1828280	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CO684_RS09650	1828400..1829401	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CO684_RS09660	1829751..1830113	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CO684_RS09665	1830110..1830280	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CO684_RS09670	1830365..1831513	-	1149	WP_096753293.1	alanine racemase	-
CO684_RS09675	1831579..1831938	-	360	WP_000581200.1	holo-ACP synthase	-
CO684_RS09680	1831942..1832433	-	492	WP_001205910.1	PH domain-containing protein	-
CO684_RS09685	1832420..1834003	-	1584	WP_001294626.1	PH domain-containing protein	-
CO684_RS09690	1833996..1834475	-	480	WP_001287088.1	hypothetical protein	-
CO684_RS09695	1834684..1835244	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T81404 WP_000621175.1 NZ_CP023500:c1830113-1829751 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T81404 NZ_CP023500:c1830113-1829751 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT81404 WP_000948331.1 NZ_CP023500:c1830280-1830110 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT81404 NZ_CP023500:c1830280-1830110 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1