Detailed information of TA system TA081393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3427969..3428624
• Replicon: chromosome
• Accession: NZ_CP023499
• Organism: Burkholderia thailandensis strain FDAARGOS_426

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CO709_RS29655
• Protein ID: WP_015600678.1
• Coordinates: 3427969..3428265 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: CO709_RS29660
• Protein ID: WP_004202809.1
• Coordinates: 3428268..3428624 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO709_RS29620	3423216..3423482	+	267	WP_015600468.1	ogr/Delta-like zinc finger family protein	-
CO709_RS29625	3423479..3423685	+	207	WP_004532731.1	ogr/Delta-like zinc finger family protein	-
CO709_RS29630	3423775..3424428	+	654	WP_015601099.1	AAA family ATPase	-
CO709_RS29635	3424662..3424784	+	123	WP_041861677.1	CopG family transcriptional regulator	-
CO709_RS29640	3424888..3425421	+	534	WP_015600440.1	PAAR domain-containing protein	-
CO709_RS29645	3425418..3426155	+	738	WP_015601730.1	VRR-NUC domain-containing protein	-
CO709_RS29650	3426170..3427285	+	1116	WP_041861678.1	DUF3396 domain-containing protein	-
CO709_RS29655	3427969..3428265	+	297	WP_015600678.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CO709_RS29660	3428268..3428624	+	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
CO709_RS29665	3428668..3429723	-	1056	WP_015600601.1	phage portal protein	-
CO709_RS29670	3429720..3431489	-	1770	WP_015602129.1	terminase ATPase subunit family protein	-
CO709_RS29675	3431683..3432120	+	438	WP_015600790.1	IS630 family transposase	-
CO709_RS29680	3432127..3432651	+	525	WP_082252628.1	transposase	-
CO709_RS29685	3432725..3433321	+	597	WP_075644826.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3414618..3456346	41728
	-	flank	IS/Tn	-	-	3431683..3432120	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10872.70 Da
• Isoelectric Point: 10.3605

>T81393 WP_015600678.1 NZ_CP023499:3427969-3428265 [Burkholderia thailandensis]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKRIAGELED

Nucleotide

• Length: 297 bp

>T81393 NZ_CP023499:3427969-3428265 [Burkholderia thailandensis]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTCGACGGGCTTCGCGACCCGGTCGGTAGCGCGGCGATCAA
CCTGCGCATCGAGCGGGCAAAGCTTGGCAATCTCGGCCAATGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCGAATCGCCGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT81393 WP_004202809.1 NZ_CP023499:3428268-3428624 [Burkholderia thailandensis]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT81393 NZ_CP023499:3428268-3428624 [Burkholderia thailandensis]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAAGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAATCCGCGCCTGATTCAAGCCGCGCTTGGGAACGTTGCGAAGGCGCGCGGCATGACGGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTACCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCACACGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3