Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF-MazE
Location 29374..29948 Replicon plasmid unnamed
Accession NZ_CP023496
Organism Staphylococcus simulans strain FDAARGOS_383

Toxin (Protein)


Gene name pemK Uniprot ID A0A6N3EA58
Locus tag CO689_RS00160 Protein ID WP_070684197.1
Coordinates 29374..29706 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A133QJA6
Locus tag CO689_RS00165 Protein ID WP_070469062.1
Coordinates 29700..29948 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO689_RS00140 26184..26618 + 435 Protein_26 protein rep -
CO689_RS00145 27292..27777 + 486 WP_070684201.1 hypothetical protein -
CO689_RS00150 28025..28243 - 219 WP_070684199.1 hypothetical protein -
CO689_RS00155 28488..28730 - 243 WP_138108225.1 hypothetical protein -
CO689_RS00160 29374..29706 - 333 WP_070684197.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CO689_RS00165 29700..29948 - 249 WP_070469062.1 hypothetical protein Antitoxin
CO689_RS13905 30798..30893 - 96 WP_138108223.1 type I toxin-antitoxin system Fst family toxin -
CO689_RS00170 31016..31346 + 331 Protein_33 IS6 family transposase -
CO689_RS00175 32178..33041 + 864 WP_096754236.1 serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaZ - 1..169687 169687


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-103)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12622.58 Da        Isoelectric Point: 9.5659

>T81387 WP_070684197.1 NZ_CP023496:c29706-29374 [Staphylococcus simulans]
MVKQFDIIMIDLDTTRGEEKQKYQPYVIISNNFVNQGSPFVWTLPITNRTKRFPSDIVVKTKRNSITGIIDSLQIKSLDI
KARNNKVVDELDESIQSDVINTIIAHSKMI

Download         Length: 333 bp

>T81387 NZ_CP023496:c29706-29374 [Staphylococcus simulans]
ATGGTAAAACAATTTGACATTATAATGATTGATCTAGATACGACTCGAGGTGAAGAGAAGCAAAAATATCAACCATATGT
AATCATAAGTAACAACTTTGTCAATCAAGGATCGCCTTTTGTTTGGACTTTACCTATAACTAATAGAACTAAGAGGTTTC
CTTCAGATATAGTTGTTAAAACAAAACGTAATAGCATAACAGGTATCATTGATTCACTACAAATAAAATCTTTAGATATT
AAAGCGAGAAATAATAAAGTAGTAGATGAATTAGATGAGAGTATCCAGTCTGATGTAATCAATACTATCATTGCACATTC
TAAAATGATTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9303.39 Da        Isoelectric Point: 5.1100

>AT81387 WP_070469062.1 NZ_CP023496:c29948-29700 [Staphylococcus simulans]
MRTLSKVIKNGNSQAISLNKSILHEAGLNIGDSLNVEINEGVVKFTKREKSIIDEIHDFYRNGGHYDEKEINFGDEVGQE
LW

Download         Length: 249 bp

>AT81387 NZ_CP023496:c29948-29700 [Staphylococcus simulans]
ATGAGAACATTATCAAAAGTTATAAAAAATGGGAACAGCCAAGCAATCAGTTTAAATAAATCCATCTTACATGAAGCAGG
TTTAAACATTGGGGATTCTTTAAATGTAGAAATAAACGAGGGCGTAGTTAAGTTTACCAAAAGAGAAAAATCAATTATCG
ATGAAATTCATGATTTTTATCGTAATGGCGGTCACTATGATGAAAAAGAAATTAATTTTGGAGATGAAGTAGGTCAAGAG
TTATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6N3EA58

Antitoxin

Source ID Structure
AlphaFold DB A0A133QJA6

References