Detailed information of TA system TA081386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 1046067..1046629
• Replicon: chromosome
• Accession: NZ_CP023490
• Organism: Lactiplantibacillus plantarum strain NCIMB 700965

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F9URY0
• Locus tag: CO218_RS05365
• Protein ID: WP_011101950.1
• Coordinates: 1046067..1046414 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CO218_RS05370
• Protein ID: WP_063851726.1
• Coordinates: 1046414..1046629 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO218_RS05330	1041350..1042207	-	858	WP_063724912.1	SDR family oxidoreductase	-
CO218_RS05335	1042647..1042868	-	222	WP_003644840.1	hypothetical protein	-
CO218_RS05340	1043124..1043567	-	444	WP_063851720.1	universal stress protein	-
CO218_RS05345	1043639..1044208	-	570	WP_003642166.1	sugar O-acetyltransferase	-
CO218_RS05350	1044305..1044988	+	684	WP_063851721.1	YjjG family noncanonical pyrimidine nucleotidase	-
CO218_RS05355	1045130..1045645	-	516	WP_063851722.1	hypothetical protein	-
CO218_RS05365	1046067..1046414	-	348	WP_011101950.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CO218_RS05370	1046414..1046629	-	216	WP_063851726.1	hypothetical protein	Antitoxin
CO218_RS05380	1048104..1048871	-	768	WP_063851724.1	hypothetical protein	-
CO218_RS05385	1048950..1050233	-	1284	WP_063851725.1	DUF1906 domain-containing protein	-
CO218_RS05390	1050360..1051535	+	1176	WP_120326207.1	IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1045130..1057824	12694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13274.06 Da
• Isoelectric Point: 9.5016

>T81386 WP_011101950.1 NZ_CP023490:c1046414-1046067 [Lactiplantibacillus plantarum]
MTYLPKQKDIIWIDFDPQRGREIKKRRPAVVLSSNLYTQNTGFVIVSPITSTMRDLPGYFSLNGYNTHGQIAAAQIYSFD
ATPRAGRSITYIETMRSADFYHVAQTVYYNFDFPF

Nucleotide

• Length: 348 bp

>T81386 NZ_CP023490:c1046414-1046067 [Lactiplantibacillus plantarum]
ATGACTTATTTACCTAAACAAAAAGACATCATCTGGATTGATTTTGACCCACAACGTGGCCGCGAAATAAAGAAACGCCG
GCCAGCTGTAGTTCTTAGTAGTAATCTCTACACTCAGAACACCGGGTTTGTAATTGTTTCACCAATTACGTCTACAATGC
GTGATTTGCCCGGCTACTTTAGTCTTAATGGTTACAATACCCACGGACAAATTGCTGCTGCACAAATTTATTCGTTTGAT
GCGACCCCCAGAGCAGGACGGAGTATCACATACATCGAAACCATGCGCAGTGCCGATTTTTATCACGTCGCGCAAACGGT
GTATTACAACTTCGACTTTCCATTCTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8032.08 Da
• Isoelectric Point: 8.5318

>AT81386 WP_063851726.1 NZ_CP023490:c1046629-1046414 [Lactiplantibacillus plantarum]
IEVKTVSRGNSLALSLPKDGRFKKGQRWLLIPSSDGESYTLVPRIENPYAGSKPKRPMTEAWSDVDWNEVE

Nucleotide

• Length: 216 bp

>AT81386 NZ_CP023490:c1046629-1046414 [Lactiplantibacillus plantarum]
ATTGAGGTAAAGACAGTTAGTCGTGGTAATTCATTGGCATTAAGTCTACCTAAAGACGGTCGTTTTAAGAAAGGACAACG
ATGGTTACTCATCCCATCAAGTGATGGTGAGTCATATACACTCGTACCACGTATCGAGAATCCTTATGCCGGCTCCAAGC
CGAAACGGCCAATGACTGAAGCATGGTCCGATGTCGACTGGAATGAGGTCGAGTAA

Structures

Toxin

• AlphaFold DB: F9URY0