Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 120043..120568 Replicon plasmid p19-10_02
Accession NZ_CP023489
Organism Klebsiella pneumoniae subsp. pneumoniae strain ST101:960186733

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag AN663_RS29890 Protein ID WP_025714517.1
Coordinates 120263..120568 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag AN663_RS29885 Protein ID WP_001568025.1
Coordinates 120043..120261 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN663_RS29845 115681..116205 - 525 Protein_124 IS5-like element ISKpn26 family transposase -
AN663_RS29850 116241..117680 - 1440 Protein_125 IS66 family transposase -
AN663_RS29855 117745..118449 + 705 WP_001572363.1 IS6-like element IS26 family transposase -
AN663_RS29860 118535..118675 + 141 WP_045286941.1 helix-turn-helix domain-containing protein -
AN663_RS29865 118773..119189 - 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin -
AN663_RS29870 119186..119416 - 231 WP_021312767.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AN663_RS29885 120043..120261 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AN663_RS29890 120263..120568 + 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
AN663_RS29895 120737..121132 + 396 WP_017899885.1 hypothetical protein -
AN663_RS29900 121159..121473 + 315 WP_053389906.1 hypothetical protein -
AN663_RS29905 121484..122500 + 1017 WP_017899884.1 hypothetical protein -
AN663_RS29910 122698..123492 + 795 WP_004197635.1 site-specific integrase -
AN663_RS30885 123972..124274 - 303 WP_071571079.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..124323 124323


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T81385 WP_025714517.1 NZ_CP023489:120263-120568 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T81385 NZ_CP023489:120263-120568 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT81385 WP_001568025.1 NZ_CP023489:120043-120261 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT81385 NZ_CP023489:120043-120261 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References