Detailed information of TA system TA081384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 118773..119416
• Replicon: plasmid p19-10_02
• Accession: NZ_CP023489
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain ST101:960186733

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A5P6KDG5
• Locus tag: AN663_RS29865
• Protein ID: WP_021312768.1
• Coordinates: 118773..119189 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A5P6KGW8
• Locus tag: AN663_RS29870
• Protein ID: WP_021312767.1
• Coordinates: 119186..119416 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN663_RS29835	114937..115401	+	465	WP_001243598.1	isoprenylcysteine carboxylmethyltransferase family protein	-
AN663_RS29845	115681..116205	-	525	Protein_124	IS5-like element ISKpn26 family transposase	-
AN663_RS29850	116241..117680	-	1440	Protein_125	IS66 family transposase	-
AN663_RS29855	117745..118449	+	705	WP_001572363.1	IS6-like element IS26 family transposase	-
AN663_RS29860	118535..118675	+	141	WP_045286941.1	helix-turn-helix domain-containing protein	-
AN663_RS29865	118773..119189	-	417	WP_021312768.1	type II toxin-antitoxin system VapC family toxin	Toxin
AN663_RS29870	119186..119416	-	231	WP_021312767.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AN663_RS29885	120043..120261	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
AN663_RS29890	120263..120568	+	306	WP_025714517.1	type II toxin-antitoxin system toxin CcdB	-
AN663_RS29895	120737..121132	+	396	WP_017899885.1	hypothetical protein	-
AN663_RS29900	121159..121473	+	315	WP_053389906.1	hypothetical protein	-
AN663_RS29905	121484..122500	+	1017	WP_017899884.1	hypothetical protein	-
AN663_RS29910	122698..123492	+	795	WP_004197635.1	site-specific integrase	-
AN663_RS30885	123972..124274	-	303	WP_071571079.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..124323	124323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15175.60 Da
• Isoelectric Point: 7.2452

>T81384 WP_021312768.1 NZ_CP023489:c119189-118773 [Klebsiella pneumoniae subsp. pneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Nucleotide

• Length: 417 bp

>T81384 NZ_CP023489:c119189-118773 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGTGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCGGCCGGCGCCGTACTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8722.81 Da
• Isoelectric Point: 4.4692

>AT81384 WP_021312767.1 NZ_CP023489:c119416-119186 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT81384 NZ_CP023489:c119416-119186 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGGTCGTTCGCGCAGCTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A5P6KDG5

Antitoxin

• AlphaFold DB: A0A5P6KGW8