Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 104564..105300 Replicon plasmid p19-10_01
Accession NZ_CP023488
Organism Klebsiella pneumoniae subsp. pneumoniae strain ST101:960186733

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AN663_RS28495 Protein ID WP_003026803.1
Coordinates 104818..105300 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AN663_RS28490 Protein ID WP_003026799.1
Coordinates 104564..104830 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN663_RS28445 100626..100988 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
AN663_RS28450 101038..101388 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AN663_RS28455 101746..102015 + 270 WP_004152102.1 hypothetical protein -
AN663_RS28460 102003..102578 + 576 WP_004152103.1 hypothetical protein -
AN663_RS28465 102609..103103 + 495 WP_004152104.1 DNA-binding protein -
AN663_RS28470 103147..103515 + 369 WP_004152105.1 hypothetical protein -
AN663_RS28475 103549..103752 + 204 WP_004152106.1 hemolysin expression modulator Hha -
AN663_RS28480 103801..104058 + 258 WP_004152107.1 hypothetical protein -
AN663_RS28485 104134..104388 + 255 WP_004152108.1 hypothetical protein -
AN663_RS28490 104564..104830 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AN663_RS28495 104818..105300 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AN663_RS28500 105512..106858 + 1347 WP_020314316.1 ISNCY family transposase -
AN663_RS28505 107359..108613 - 1255 Protein_111 IS3 family transposase -
AN663_RS28510 108701..109663 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / tet(D) / blaNDM-1 / sul1 / armA / msr(E) / mph(E) / aph(6)-Id / aph(3'')-Ib / sul2 - 1..223434 223434


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T81383 WP_003026803.1 NZ_CP023488:104818-105300 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T81383 NZ_CP023488:104818-105300 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT81383 WP_003026799.1 NZ_CP023488:104564-104830 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT81383 NZ_CP023488:104564-104830 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References