Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4727447..4728066 Replicon chromosome
Accession NZ_CP023487
Organism Klebsiella pneumoniae subsp. pneumoniae strain ST101:960186733

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AN663_RS24050 Protein ID WP_002892050.1
Coordinates 4727848..4728066 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag AN663_RS24045 Protein ID WP_002892066.1
Coordinates 4727447..4727821 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN663_RS24035 4722599..4723792 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AN663_RS24040 4723815..4726961 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
AN663_RS24045 4727447..4727821 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
AN663_RS24050 4727848..4728066 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AN663_RS24055 4728225..4728791 + 567 WP_002892030.1 maltose O-acetyltransferase -
AN663_RS30790 4728763..4728903 - 141 WP_004147370.1 hypothetical protein -
AN663_RS24060 4728924..4729394 + 471 WP_002892026.1 YlaC family protein -
AN663_RS24065 4729369..4730820 - 1452 WP_055314367.1 PLP-dependent aminotransferase family protein -
AN663_RS24070 4730921..4731619 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
AN663_RS24075 4731616..4731756 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
AN663_RS24080 4731756..4732019 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T81382 WP_002892050.1 NZ_CP023487:4727848-4728066 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T81382 NZ_CP023487:4727848-4728066 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT81382 WP_002892066.1 NZ_CP023487:4727447-4727821 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT81382 NZ_CP023487:4727447-4727821 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References