Detailed information of TA system TA081371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2296728..2297318
• Replicon: chromosome
• Accession: NZ_CP023487
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain ST101:960186733

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: AN663_RS11535
• Protein ID: WP_023341911.1
• Coordinates: 2296986..2297318 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: AN663_RS11530
• Protein ID: WP_000288812.1
• Coordinates: 2296728..2296985 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN663_RS11505	2293063..2294811	+	1749	WP_057171892.1	hypothetical protein	-
AN663_RS11510	2294865..2295446	+	582	WP_099184326.1	hypothetical protein	-
AN663_RS11515	2295729..2296190	+	462	WP_004213450.1	hypothetical protein	-
AN663_RS11520	2296187..2296393	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
AN663_RS11530	2296728..2296985	+	258	WP_000288812.1	antitoxin	Antitoxin
AN663_RS11535	2296986..2297318	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AN663_RS11545	2297640..2299076	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
AN663_RS11555	2299442..2300896	-	1455	WP_004148975.1	AMP nucleosidase	-
AN663_RS11560	2301026..2301271	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T81371 WP_023341911.1 NZ_CP023487:2296986-2297318 [Klebsiella pneumoniae subsp. pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T81371 NZ_CP023487:2296986-2297318 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT81371 WP_000288812.1 NZ_CP023487:2296728-2296985 [Klebsiella pneumoniae subsp. pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT81371 NZ_CP023487:2296728-2296985 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0