Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 147897..148432 Replicon plasmid pKQPS142a
Accession NZ_CP023479
Organism Klebsiella quasipneumoniae strain KPC142

Toxin (Protein)


Gene name relE Uniprot ID A0A2A5MBV5
Locus tag KPC142_RS26760 Protein ID WP_032420954.1
Coordinates 148145..148432 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2A5MCI9
Locus tag KPC142_RS26755 Protein ID WP_032420955.1
Coordinates 147897..148148 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPC142_RS26730 143656..143886 - 231 WP_004206779.1 hypothetical protein -
KPC142_RS26735 144120..145631 - 1512 WP_024623221.1 group II intron reverse transcriptase/maturase -
KPC142_RS26745 146034..146459 + 426 WP_001776124.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
KPC142_RS26750 146459..147730 + 1272 WP_032420957.1 Y-family DNA polymerase -
KPC142_RS26755 147897..148148 + 252 WP_032420955.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KPC142_RS26760 148145..148432 + 288 WP_032420954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KPC142_RS26765 148536..149233 + 698 Protein_165 IS1 family transposase -
KPC142_RS26770 149454..150851 + 1398 WP_032452481.1 diguanylate cyclase -
KPC142_RS26775 151004..151753 - 750 Protein_167 tryptophan--tRNA ligase -
KPC142_RS26780 151768..152193 - 426 Protein_168 IS1-like element IS1B family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..189707 189707


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.13 Da        Isoelectric Point: 10.2246

>T81338 WP_032420954.1 NZ_CP023479:148145-148432 [Klebsiella quasipneumoniae]
MTYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDHLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T81338 NZ_CP023479:148145-148432 [Klebsiella quasipneumoniae]
ATGACTTATACGGTCAAATTCAGGGAAGATGCTCTGAAAGAATGGCAAAAGCTGGATAAGGCCATTCAGCAGCAGTTTGC
GAAGAAACTGAAGAAGTGCTGTGACGAACCGCATATCCCCTCAGCAAAGCTGAGAGGGATAAAGGACTGCTATAAAATTA
AACTTCGTGCATCTGGTTTTCGCCTAGTATATCAGGTGATAGATGATCATTTAATTATCGCAGTTGTTGCTGTAGGAAAG
CGTGAGCGTAGTGACGTGTACAATCTGGCCAGCGAAAGGATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9192.47 Da        Isoelectric Point: 3.9507

>AT81338 WP_032420955.1 NZ_CP023479:147897-148148 [Klebsiella quasipneumoniae]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDAMDDQELAKLVSERSNQQLLDVDLD
TYL

Download         Length: 252 bp

>AT81338 NZ_CP023479:147897-148148 [Klebsiella quasipneumoniae]
ATGCCCAACATTATTCTGAGTGACACAAGCGCGAGCATAAGCGAACTTAAAAAAAACCCGATGGCGACAGTCAGTGCCGG
CGACGGCTTCCCGGTCGCTATTCTGAACCGTAACCAACCCGCTTTTTACTGTATCCCGGCAGAGCTTTACGAGAAGATGC
TTGATGCCATGGATGATCAGGAACTGGCAAAGCTGGTTTCAGAACGCAGTAATCAGCAGCTGCTTGACGTGGATCTGGAC
ACTTATCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBV5

Antitoxin

Source ID Structure
AlphaFold DB A0A2A5MCI9

References