Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 112323..113059 Replicon plasmid pKQPS142a
Accession NZ_CP023479
Organism Klebsiella quasipneumoniae strain KPC142

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KPC142_RS26485 Protein ID WP_003026803.1
Coordinates 112323..112805 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KPC142_RS26490 Protein ID WP_003026799.1
Coordinates 112793..113059 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPC142_RS26465 107619..108323 - 705 WP_031591821.1 toll/interleukin-1 receptor domain-containing protein -
KPC142_RS26470 108483..109829 - 1347 WP_077251107.1 ISNCY family transposase -
KPC142_RS26475 110058..110690 + 633 WP_001567369.1 hypothetical protein -
KPC142_RS26480 110719..112122 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
KPC142_RS26485 112323..112805 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KPC142_RS26490 112793..113059 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KPC142_RS26500 113504..113734 - 231 WP_004206660.1 hypothetical protein -
KPC142_RS26505 113748..113951 - 204 WP_004206661.1 hemolysin expression modulator Hha -
KPC142_RS26510 114012..114503 - 492 WP_004206662.1 hypothetical protein -
KPC142_RS26515 114637..115848 - 1212 WP_032415728.1 hypothetical protein -
KPC142_RS26520 115845..116093 - 249 WP_004193994.1 hypothetical protein -
KPC142_RS26525 116451..116801 + 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KPC142_RS26530 116853..117215 + 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..189707 189707


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T81337 WP_003026803.1 NZ_CP023479:c112805-112323 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T81337 NZ_CP023479:c112805-112323 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT81337 WP_003026799.1 NZ_CP023479:c113059-112793 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT81337 NZ_CP023479:c113059-112793 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References