Detailed information of TA system TA081336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 34832..35742
• Replicon: plasmid pKQPS142a
• Accession: NZ_CP023479
• Organism: Klebsiella quasipneumoniae strain KPC142

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2A5MBW7
• Locus tag: KPC142_RS26070
• Protein ID: WP_023329005.1
• Coordinates: 34832..35302 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2A5MC16
• Locus tag: KPC142_RS26075
• Protein ID: WP_032731962.1
• Coordinates: 35299..35742 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPC142_RS26040	29857..30239	+	383	Protein_34	transposase	-
KPC142_RS26050	30516..31484	-	969	WP_022652343.1	IS5-like element IS903B family transposase	-
KPC142_RS26055	31568..32143	+	576	WP_032731970.1	cytochrome b/b6 domain-containing protein	-
KPC142_RS26065	34462..34721	+	260	Protein_38	hypothetical protein	-
KPC142_RS26070	34832..35302	-	471	WP_023329005.1	RES family NAD+ phosphorylase	Toxin
KPC142_RS26075	35299..35742	-	444	WP_032731962.1	DUF2384 domain-containing protein	Antitoxin
KPC142_RS27575	35864..36551	-	688	Protein_41	DUF4113 domain-containing protein	-
KPC142_RS26085	36712..37707	-	996	WP_032731961.1	DUF2891 domain-containing protein	-
KPC142_RS26090	37717..38703	-	987	Protein_43	DUF979 domain-containing protein	-
KPC142_RS26095	38700..39421	-	722	Protein_44	DUF969 domain-containing protein	-
KPC142_RS26100	39620..39958	+	339	WP_032731959.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..189707	189707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17459.70 Da
• Isoelectric Point: 4.4829

>T81336 WP_023329005.1 NZ_CP023479:c35302-34832 [Klebsiella quasipneumoniae]
VILYRLTKTKNLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPANWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHSDFYGIVQMAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T81336 NZ_CP023479:c35302-34832 [Klebsiella quasipneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGAATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAATTGATCCAAAGCGCAAATATGGAT
GAATTACCGGCCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCTCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACTCTGATTTTT
ACGGGATCGTGCAGATGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16424.76 Da
• Isoelectric Point: 10.2497

>AT81336 WP_032731962.1 NZ_CP023479:c35742-35299 [Klebsiella quasipneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAALKWLNEPNRALSWKVPADLIASETGAYEVIKLITRLENGVYS

Nucleotide

• Length: 444 bp

>AT81336 NZ_CP023479:c35742-35299 [Klebsiella quasipneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTGCTGGGTCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AAAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGACTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGATAAGGAAGCCGCACTGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATAGCATCCGAGACGGGAGCATATGAGG
TCATTAAGCTGATTACGCGTCTGGAGAATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MBW7

Antitoxin

• AlphaFold DB: A0A2A5MC16