Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3866389..3867008 Replicon chromosome
Accession NZ_CP023478
Organism Klebsiella quasipneumoniae strain KPC142

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KPC142_RS19220 Protein ID WP_002892050.1
Coordinates 3866790..3867008 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KPC142_RS19215 Protein ID WP_002892066.1
Coordinates 3866389..3866763 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPC142_RS19205 3861543..3862736 + 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KPC142_RS19210 3862759..3865905 + 3147 WP_004204748.1 multidrug efflux RND transporter permease subunit -
KPC142_RS19215 3866389..3866763 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KPC142_RS19220 3866790..3867008 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
KPC142_RS19225 3867166..3867732 + 567 WP_004204750.1 maltose O-acetyltransferase -
KPC142_RS27505 3867704..3867826 - 123 WP_032426076.1 hypothetical protein -
KPC142_RS19230 3867869..3868339 + 471 WP_004204751.1 YlaC family protein -
KPC142_RS19235 3868308..3869765 - 1458 WP_049117056.1 PLP-dependent aminotransferase family protein -
KPC142_RS19240 3869866..3870564 + 699 WP_004204753.1 GNAT family N-acetyltransferase -
KPC142_RS19245 3870561..3870701 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KPC142_RS19250 3870701..3870964 - 264 WP_004204754.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T81335 WP_002892050.1 NZ_CP023478:3866790-3867008 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T81335 NZ_CP023478:3866790-3867008 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT81335 WP_002892066.1 NZ_CP023478:3866389-3866763 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT81335 NZ_CP023478:3866389-3866763 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References