Detailed information of TA system TA081291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09812-MazE
• Location: 6093387..6094018
• Replicon: chromosome
• Accession: NZ_CP023466
• Organism: Pseudomonas frederiksbergensis strain KNU-15

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CNN82_RS27940
• Protein ID: WP_096481349.1
• Coordinates: 6093387..6093749 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A291APG8
• Locus tag: CNN82_RS27945
• Protein ID: WP_019581886.1
• Coordinates: 6093749..6094018 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNN82_RS27930	6088518..6091868	+	3351	WP_096481347.1	mechanosensitive channel MscK	-
CNN82_RS27935	6091917..6093380	+	1464	WP_028939627.1	YdiU family protein	-
CNN82_RS27940	6093387..6093749	-	363	WP_096481349.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CNN82_RS27945	6093749..6094018	-	270	WP_019581886.1	PbsX family transcriptional regulator	Antitoxin
CNN82_RS27950	6094288..6094722	+	435	WP_019581885.1	thioredoxin TrxC	-
CNN82_RS27955	6094730..6095500	-	771	WP_018928585.1	ParA family protein	-
CNN82_RS27960	6095857..6096582	+	726	WP_019581883.1	beta-ketoacyl synthase chain length factor	-
CNN82_RS27965	6096558..6097370	+	813	WP_018928587.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
CNN82_RS27970	6097348..6097623	+	276	WP_018928588.1	acyl carrier protein	-
CNN82_RS27975	6097633..6097887	+	255	WP_007936741.1	acyl carrier protein	-
CNN82_RS27980	6097884..6098429	+	546	WP_019581882.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13222.09 Da
• Isoelectric Point: 7.1674

>T81291 WP_096481349.1 NZ_CP023466:c6093749-6093387 [Pseudomonas frederiksbergensis]
MVRRQAPQRGDVYWIDPNPVAGREMMNRHRFVVITPREINVLGVSMTVPVTSGGSFSRNSGLAVIVSGHETNGVAVCNQV
RSFDIEQRVRDGSAKFIERLDDVTMADIVARVVSAIDPLD

Nucleotide

• Length: 363 bp

>T81291 NZ_CP023466:c6093749-6093387 [Pseudomonas frederiksbergensis]
ATGGTGCGTCGACAAGCCCCGCAGCGGGGCGATGTCTATTGGATTGATCCGAATCCGGTCGCAGGACGAGAGATGATGAA
TCGTCATCGCTTCGTGGTGATAACGCCAAGGGAAATCAACGTACTGGGTGTCAGCATGACTGTACCCGTGACCAGTGGCG
GGAGTTTCAGTCGAAATTCAGGTCTGGCGGTTATCGTGTCCGGCCACGAAACAAACGGCGTTGCAGTGTGTAATCAGGTA
CGCAGTTTTGACATTGAGCAACGTGTTCGCGATGGTTCGGCCAAGTTTATCGAACGACTTGATGACGTCACGATGGCAGA
CATCGTCGCTCGAGTCGTCAGCGCCATAGATCCTCTGGATTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9630.15 Da
• Isoelectric Point: 5.1069

>AT81291 WP_019581886.1 NZ_CP023466:c6094018-6093749 [Pseudomonas frederiksbergensis]
MSVTSKIRRQGGAAVITLPPTLLKLLHLEVGAQLELNVVDGELIARPVIQPPRKRYSLEELLEGSEAMAELNAQTAFARE
GDPVGRELG

Nucleotide

• Length: 270 bp

>AT81291 NZ_CP023466:c6094018-6093749 [Pseudomonas frederiksbergensis]
ATGTCCGTGACCTCCAAAATCCGCCGACAAGGCGGCGCAGCGGTGATTACTCTTCCGCCTACCTTATTGAAGCTCCTGCA
CCTGGAGGTGGGGGCACAATTGGAATTGAATGTGGTGGATGGCGAGTTGATAGCTCGTCCGGTTATCCAGCCGCCTCGCA
AGCGTTACAGCCTTGAAGAGTTGCTTGAGGGCTCCGAAGCAATGGCCGAATTGAACGCACAGACAGCCTTTGCGCGTGAA
GGCGATCCAGTCGGCCGGGAGCTCGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A291APG8